Electrospray Ionization Mass Spectrometric Analysis of Proteins
Electrospray is a ‘soft’ ionization technique in which intact gas phase ions of involatile and thermally labile biomolecules are produced directly from an analyte solution of interest at atmospheric pressure. Electrospray occurs when a strong electric field is applied to a small flow of liquid emerging through a fine capillary tube. The strong electric field causes the surface of the emerging liquid to become highly charged resulting in the formation of a fine spray of charged droplets. The solvents continuously evaporate from the droplets as the droplets proceed from atmospheric pressure to the vacuum leading to the formation of gas phase solute ions.
KeywordsCapillary Tube Strong Electric Field Human Hemoglobin Electro Spray Ionization Quadrupole Analyzer
Unable to display preview. Download preview PDF.
- 4.V. Katta, S.K. Chowdhury, and B.T. Chait, Anal Chem. (submitted).Google Scholar
- 9.J. Darnell, H. Lodish, and D. Baltimore, Molecular Cell Biology, W.H. Freeman, NY, 1986, pp. 57–9.Google Scholar
- 10.S.K. Chowdhury, V. Katta, and B.T. Chait, J. Am. Chem. Soc. (submitted).Google Scholar
- 12.Protein Identification Resource (PIR), National Biomedical Research Foundation (NBRF) Release 19.0 (3187 December 1988).Google Scholar
- 14a.E. Gross and B. Witkop, Biochem. 6 (1967) 745.Google Scholar