Sequencing an Unknown Peptide
For a recent American Society for Mass Spectrometry (ASMS) peptide sequencing workshop, some forty laboratories requested a sample of two unknown peptides. We chose to analyse a 1.5 μg sample with a stated mass of ~ 1800 u, and were the only laboratory to submit results on this peptide at the 1990 ASMS conference in Tucuon, Arizona.
KeywordsSide Chain Fragmentation Carboxyl Side Unknown Peptide Aqueous Trifluoroacetic Acid Chymotryptic Digest
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- 3.B. T. Chait, T. Chaudhury, F. H. Field, in:”Methods in Protein Sequence Analysis 1986”, Ed. K. A. Walsh, Humana Press, Clifton, New Jersey (1987) p.483.Google Scholar