Advertisement

Sequencing an Unknown Peptide

  • N. Poppe-Schriemer
  • D. R. Binding
  • W. Ens
  • J. C. Martens
  • F. Mayer
  • K. G. Standing
  • X. Tang
  • J. B. Westmore
Part of the NATO ASI Series book series (NSSB, volume 269)

Abstract

For a recent American Society for Mass Spectrometry (ASMS) peptide sequencing workshop, some forty laboratories requested a sample of two unknown peptides. We chose to analyse a 1.5 μg sample with a stated mass of ~ 1800 u, and were the only laboratory to submit results on this peptide at the 1990 ASMS conference in Tucuon, Arizona.

Keywords

Side Chain Fragmentation Carboxyl Side Unknown Peptide Aqueous Trifluoroacetic Acid Chymotryptic Digest 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    X. Tang, R. Beavis, W. Ens, F. Lafortune, B. Schueler and K. G. Standing, Int. J. Mass Spectrom. Ion Proc. 85, (1988) 43.CrossRefGoogle Scholar
  2. 2.
    F. Lafortune, R. Beavis, X. Tang, K. G. Standing, and B. T. Chait, Rapid Comm. Mass Spectrom. 1,(1987) 114.CrossRefGoogle Scholar
  3. 3.
    B. T. Chait, T. Chaudhury, F. H. Field, in:”Methods in Protein Sequence Analysis 1986”, Ed. K. A. Walsh, Humana Press, Clifton, New Jersey (1987) p.483.Google Scholar
  4. 4.
    P. Roepstorff and J. Fohlman, Biomed. Mass Spectrom. 11, (1984) 601.CrossRefGoogle Scholar
  5. 5.
    K. Biemann, Biomed. Environ. Mass Spectrom. 16, (1988) 99.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • N. Poppe-Schriemer
    • 1
  • D. R. Binding
    • 1
  • W. Ens
    • 1
  • J. C. Martens
    • 1
  • F. Mayer
    • 1
  • K. G. Standing
    • 1
  • X. Tang
    • 1
  • J. B. Westmore
    • 2
  1. 1.Departments of PhysicsUniversity of ManitobaWinnipegCanada
  2. 2.Departments of ChemistryUniversity of ManitobaWinnipegCanada

Personalised recommendations