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Silver Impregnation of Degenerating Axons in the Central Nervous System: A Modified Technic

  • W. J. H. Nauta
  • P. A. Gygax
Part of the Contemporary Neuroscientists book series (CN)

Abstract

Frozen sections of formalin-fixed brains containing surgical lesions, were treated with 15% ethanol for 0.5 hr., soaked in 0.5% phosphomolybdic acid for 0.25–1.0 hr., and subsequently treated with 0.05% potassium permanganate for 4–10 min. (The duration of the latter treatment is critical and individually variable). Subsequent procedure is as follows: decolorize in a mixture of equal parts of 1% hydroquinone and 1% oxalic acid; wash thoroughly and soak sections in 1.5% silver nitrate for 20–30 min.; ammoniacal silver nitrate (silver nitrate 0.9 g., distilled water 20 ml., pure ethanol 10 ml., strong ammonia 1.8 ml., 2.5% sodium hydroxide 1.5 ml.) 0.5–1.0 min.; reduce in acidified formalin (distilled water 400 ml., pure ethanol 45 ml., 1% citric acid 13.5 ml., 10% formalin 13.5 ml.) 1 min.; wash, and pass section through 1% sodium thiosulfate (0.5–1.0 min.); wash thoroughly and pass sections through graded alcohols and xylene (3 changes); cover in neutral synthetic resin.

Keywords

Oxalic Acid Silver Nitrate Axon Degeneration Potassium Permanganate Pure Ethanol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Nauta, W. J. H., and Ryan, L. F. 1952. Selective silver impregnation of degenerating axons in the central nervous system. Stain Techn., 27, 175–9.Google Scholar
  2. Albrecht, M. H. 1954. Mounting frozen sections with gelatin. Stain Techn., 29, 89–90.Google Scholar

Copyright information

© Birkhäuser Boston 1993

Authors and Affiliations

  • W. J. H. Nauta
    • 1
  • P. A. Gygax
    • 1
  1. 1.Army Medical Service Graduate School and Department of BiochemistryGeorgetown University Medical SchoolUSA

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