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Enzymes Covalently Bound on Collagen Membranes Immobilization of Blood Clotting Factor XIII

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Biotechnological Applications of Lipid Microstructures

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 238))

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Abstract

Ever since 1958 I have specialized in the field of mitochondrial research: metabolism, structure of inner mitochondrial membrane, ATP synthase. Therefore one can wonder why I started binding enzymes to collagen membranes and why we specially immobilized Factor XIII in this type of thin membranes, since we had no competence concerning blood clotting in the Laboratory. In fact, in a first approach, with J.H. Julliard, hand-made ultra-thin films of collagen were prepared in the Laboratory and used for the covalent binding of glutamate dehydrogenase by an azide method to make a simple model of interactions of glutamate dehydrogenase with inner mitochondrial membrane1. Glutamate dehydrogenase is an abundant mitochondrial enzyme that strongly interacts specifically with the inner face of the inner mitochondrial membrane, therefore from the matrix side. If the kinetic parameters of the enzyme were somewhat modified, however, the bound glutamate dehydrogenase kept its cooperative properties and regulations by the effectors ADP and GTP. This meant that the bound enzyme had kept its specific oligomeric structure and organization. It was such a success that we decided to develop the system.

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Authors and Affiliations

  1. Laboratoire de Biologie et Technologie des Membranes, CNRS — Université Claude Bernard de Lyon, 69622, Villeurbanne Cedex, France

    Danièle C. Gautheron, Bruno G. Blanchy & Pierre R. Coulet

Authors
  1. Danièle C. Gautheron
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  2. Bruno G. Blanchy
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  3. Pierre R. Coulet
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Editors and Affiliations

  1. Naval Research Laboratory, Washington, D.C., USA

    Bruce Paul Gaber  & Joel M. Schnur  & 

  2. Royal Free Hospital School of Medicine, London, UK

    Dennis Chapman

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© 1988 Plenum Press, New York

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Gautheron, D.C., Blanchy, B.G., Coulet, P.R. (1988). Enzymes Covalently Bound on Collagen Membranes Immobilization of Blood Clotting Factor XIII. In: Gaber, B.P., Schnur, J.M., Chapman, D. (eds) Biotechnological Applications of Lipid Microstructures. Advances in Experimental Medicine and Biology, vol 238. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-7908-9_26

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  • DOI: https://doi.org/10.1007/978-1-4684-7908-9_26

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