Development of Plastid Membranes in Immobilized Systems
The plant cell is characterized by chloroplasts, the cell wall and a large vacuole. Due to their size and the presence of the large vacuole, isolated plant protoplasts are often fragile. The metabolic production of several expensive chemicals in plant cells has made the development of industrially useful systems interesting (Misawa and Suzuki, 1982; Fowler, 1983). The fragility of the plant protoplast and the tendency for newly synthesized cell walls to aggregate complicates the use of plant cell cultures for industrial purposes (Lindsey and Yeoman, 1983). Immobilizing plant protoplasts would be a clear improvement (Brodelius and Nilsson, 1980). It might, however, be even more advantageous to immobilize plant organells or membrane systems which can perform suitable biosynthetic pathways. The stability of immobilized membrane systems is poorly known and a possible in vitro differentiation of the membrane systems is not fully understood. The chloroplasts are characterized by a well-differentiated inner membrane system made up of thylakoids. A differentiation of this system could be a suitable model system.
KeywordsChlorophyll Agar Epoxy Tungsten Shrinkage
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