Artificial Cells with Ultrathin Lipid-Polymer or Lipid-Protein Membranes

  • T. M. S. Chang
Part of the Advances in Experimental Medicine and Biology book series (AEMB)


Cell membranes consist of phospholipid-cholesterol bilayer with other components incorporated. In addition, there is a “skeletal” protein structure supporting the bilayer. This “skeletal” protein structure is very important in the case of red blood cells. It retains the shape and integrity of the red blood cell membrane. The first artificial cells prepared have membranes of cross-linked protein or synthetic polymers (Chang, 1957, 1964). Later, a single bilayer of phospholipid-cholesterol is added to the outside of the cross-linked protein membrane or polymer membrane (Chang, 1969a, 1972b). These artificial cells with lipid-protein membrane resembles more closely biological cells. The use of ultrathin lipid-polymer membranes for artificial cells results in stronger membranes. The flux for sodium and rubidium across these artificial cells is the same as for red blood cells (Chang, 1969a, 1972b, Rosenthal and Chang, 1980). Furthermore, addition of valinomycin to the suspension increases influx for rubidium without any change in sodium influx (Rosenthal and Chang, 1980). The following is an updated method for the preparation of lipid-polymer membrane artificial cells (Yu and Chang, 1982; Chang, 1985, 1987, Ilan and Chang, 1986).


Immobilize Enzyme Glucose Dehydrogenase Artificial Cell Blood Substitute Multienzyme System 


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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • T. M. S. Chang
    • 1
  1. 1.Artificial Cells and Organs Research Centre Faculty of MedicineMcGill UniversityMontrealCanada

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