Liposome-Based Immunoassays for Detection of Small and Large Molecules
Liposomes provide a convenient mechanism for encapsulating reporter molecules for signal amplification. This manuscript describes detection systems built by attaching a liposome filled with fluorophores to a recognition unit. The fluorophore, carboxyfluorescein (CF), is encapsulated at a high concentration so that it is self-quenched and exhibits low fluorescence. Upon activation of the recognition unit, the liposome is lysed, the CF is diluted into the medium, and a signal is generated. The detection unit is based on the formation of an antigen-antibody complex which generates lysis of the liposome subsequent to the activation of the serum complement cascade. The general scheme of this detection system has been previously described (Six et al., 1973; Yasuda et al., 1981; Martin and Papahadjopoulos, 1982; Ishimori et al., 1984).
KeywordsOxalyl Chloride Recognition Unit Rabbit Complement Dicetyl Phosphate Liposome Stability
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