Chemical-Induced Differentiation of Cultured Human Keratinocytes as Measured by Cell Size Distribution
Polyhalogenated aromatic hydrocarbons (including dibenzo-p-dioxins, dibenzofurans, biphenyls and azobenzenes) can induce alteration of the differentiation pattern of the epithelial cells in target tissues . The toxicity of polyhalogenated aromatic hydrocarbons depends on the number and position of the halogen atoms . Especially those compounds substituted on the lateral positions of the rings are able to induce a variety of toxic responses. The most common effect in all species is the wasting syndrome, marked by a reduction in body weight and adipose tissue. Thymus atrophy and hepatomegaly are frequently observed in all species after dioxin exposure. Other toxic responses, such as skin and gastric lesions, are regarded as more species specific .
KeywordsAromatic Hydrocarbon Terminal Differentiation Human Keratinocytes Minimal Effective Concentration Cell Size Distribution
Unable to display preview. Download preview PDF.
- 3.P.J.J.M. Weterings, H.M.J. Roelofs, A.J.M. Vermorken and H. Bloemendal, Serial cultivation of human hair follicle keratinocytes, Acta Dermato- Venerelogica, 63: 315 (1983)Google Scholar
- 4.J.G. Rheinwald and M.A. Beckett, Tumorgenic keratinocyte lines requiring anchorage and fibroblast support cultured from human squamous cell carcinomas, Cancer Res., 42: 1657 (1981)Google Scholar
- 6.W.F. Greenlee, R. Osborn, K.M. Dold, L.G. Hudson, M.J. Young and W.A. Toscano Jr., Altered regulation of epidermal cell proliferation and differentiation by 2,3,7,8-tetrachlorodibenzo-p-dioxin, in: “Reviews Biochemical Toxicology 8”, E. Hodgson, J.R. Bend and R.P. Philpot, eds., Elsevier, New York (1987)Google Scholar
- 7.H. Green, The keratinocyte as differentiated cell type, The Harvey Lectures Series, 74: 101 (1980)Google Scholar
- 11.Working Group Toxicity Equivalency Factors, “Proposal for a method for assessment of the toxicity of mixtures of halogenated dibenzo-p-dioxins and dibenzofurans”, Utrecht, (1988)Google Scholar