In Vitro Expanded Human Keratinocytes: Clinical and Biological Experiences
Until recently it seemed impossible to culture human keratinocytes. In 1975, the use of a feeder layer of irradiated mouse-fibroblasts in the culture of the epithelial cells opened the way to the first successes (1). The subsequent use of growth factors, especially EGF, led to increased cell yield and faster confluence (2).
KeywordsFeeder Layer Human Epidermal Keratinocytes Epithelial Sheet Artificial Skin Burnt Patient
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