In Vitro Expanded Human Keratinocytes: Clinical and Biological Experiences

  • L. Andreassi
  • L. Donati
  • M. Malcovati
  • M. L. Tenchini
  • E. Trabucchi
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 181)


Until recently it seemed impossible to culture human keratinocytes. In 1975, the use of a feeder layer of irradiated mouse-fibroblasts in the culture of the epithelial cells opened the way to the first successes (1). The subsequent use of growth factors, especially EGF, led to increased cell yield and faster confluence (2).


Feeder Layer Human Epidermal Keratinocytes Epithelial Sheet Artificial Skin Burnt Patient 
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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • L. Andreassi
    • 4
  • L. Donati
    • 2
  • M. Malcovati
    • 1
  • M. L. Tenchini
    • 1
  • E. Trabucchi
    • 3
  1. 1.Dipartimento di Biologia e Genetica per le Scienze MedicheUniversità di MilanoItaly
  2. 2.Cattedra di Chirurgia PlasticaUniversità di MilanoItaly
  3. 3.Istituto di Clinica ChirurgicaUniversità di MilanoItaly
  4. 4.Istituto di Clinica DermosifilopaticaUniversità di SienaItaly

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