Advertisement

The Use of Cultured Human Keratinocytes as a Model System for Skin Pharmacology and Toxicology

  • Uwe Reichert
  • Rainer Schmidt
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 181)

Abstract

The skin is a peripheral organ exposed to the environment where it comes frequently in contact with toxic agents to which it may respond with irritation. This response is often the consequence of a cytotoxic action on epidermal cells.

Keywords

Thymidine Incorporation Human Keratinocytes Organotypic Culture Cornified Envelope Epidermal Growth Factor Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    S. M. Fischer, A. Viaje, G. D. Mills and T. J. Slaga, Explant methods for epidermal cell culture, in: Methods in Cell Biology, vol. 21, C. C. Harris, B. F. Trump and G. D. Stoner, eds., Academic Press, New York (1980).Google Scholar
  2. 2.
    M. Pruniéras, M. Régnier and D. Woodley, Methods for cultivation of keratinocytes with an air-liquid interface, J. Invest. Dermatol. 81:28s (1983).Google Scholar
  3. 3.
    J. G. Rheinwald and H. Green, Serial cultivation of strains of human keratinocytes: The formation of keratinizing colonies from single cells, Cell 6:331 (1975).PubMedCrossRefGoogle Scholar
  4. 4.
    S.-C. Liu, M. F. Eaton and M. A. Karasek, Growth characteristics of human epidermal keratinocytes from newborn foreskin in primary and serial culture, In Vitro 15:813 (1979).Google Scholar
  5. 5.
    J. Taylor-Papadimitriou, P. Purkis, E. B. Lane, I. A. McKay and S. E. Chang, Effect of SV40 transformation on the cytoskeleton and behavioural properties of human keratinocytes. Cell Differ. 11:169 (1982).PubMedCrossRefGoogle Scholar
  6. 6.
    U. Reichert, Skin toxicity and cellular metabolism: in vitro models, Br. J. Dermatol. 115 (Suppl. 31):108 (1986)PubMedCrossRefGoogle Scholar
  7. 7.
    U. Reichert, A. Chatelus, and G. Pautrat, Use of cultured human cells as screening models for antipsoriatic drugs, Alternative Methods in Toxicology, Vol. V, A. M. Goldberg, ed., Mary Ann Liebert, New York (1987).Google Scholar
  8. 8.
    U. Reichert, Y. Jacques, M. Grangeret, and R. Schmidt, Antirespiratory and antiproliferative activity of anthralin in cultured human keratinocytes, J. Invest. Dermatol. 84:130 (1985).PubMedCrossRefGoogle Scholar
  9. 9.
    U. Reichert, and B. Shroot, Effect of antipsoriatic drugs on the basic metabolism of cultured human keratinocytes, in: Models in Dermatology, vol. 3, H. I. Maibach and N. J. Lowe, eds., Karger, Basel (1987).Google Scholar
  10. 10.
    B. Kammerau, A. Zesch and H. Schaefer, Absolute concentrations of dithranol and triacetyl-dithranol in the skin layers after local treatment: in vivo investigations with four different types of pharmacological vehicles. J. Invest. Dermatol. 64:145 (1975).PubMedCrossRefGoogle Scholar
  11. 11.
    D. Cavey, R. G. Dickinson, B. Shroot and H. Schaefer, The in vivo fate of topically applied dithranol in the skin of the hairless rat, Arzneim.-Forsch. Drug Res. 35:605 (1985).Google Scholar
  12. 12.
    J. A. Parrish, T. B. Fitzpatrick, L. Tanenbaum and M. A. Pathak, Photochemotherapy of psoriasis with oral methoxsalen and long-wave ultraviolet light, New Engl. J. Med. 291:1207 (1974).Google Scholar
  13. 13.
    J. D. Laskin, E. Lee, E. J. Yurkow, D. L. Laskin and M. A. Gallo, A possible mechanism of psoralen phototoxicity not involving direct interaction with DNA. Proc. Natl. Acad. Sci. USA 82:6158 (1985).PubMedCrossRefGoogle Scholar
  14. 14.
    J. D. Laskin, E. Lee, D. L. Laskin and M. A. Gallo, Psoralens potentiate ultraviolet light — induced inhibition of epidermal growth factor binding. Proc. Natl. Acad. Sci. USA 83:8211 (1986).PubMedCrossRefGoogle Scholar
  15. 15.
    E. Christophers and J. Pohl, Epidermal reactions to photochemotherapy, in: The Epidermis in Disease, R. Marks and E. Christophers, eds., MTP Press, Lancaster (1981).Google Scholar
  16. 16.
    T. Henseler, K. Wolf, H. Hönigsmann and E. Christophers, Oral 8-methoxypsoralen photochemotherapy of psoriasis. The European PUVA study: a cooperative study among 18 European centres, Lancet, i, 853 (1981).CrossRefGoogle Scholar
  17. 17.
    J. W. Melski, L. Tanenbaum, J. A. Parrish, T. B. Fitzpatrick, H. L. Bleich et al., Oral methoxsalen photochemotherapy for the treatment of psoriasis: a co-operative clinical trial, J. Invest. Dermatol. 68:328 (1977).PubMedCrossRefGoogle Scholar
  18. 18.
    G. Stuettgen, Zur Lokalbehandlung von Keratosen mit Vitamin-A-Säure, Dermatologica 124:65 (1962).PubMedCrossRefGoogle Scholar
  19. 19.
    B. M. Gilfix, and H. Green, Bioassay of retinoids using cultured human conjunctival keratinocytes, J. Cell. Physiol. 119:172 (1984).PubMedCrossRefGoogle Scholar
  20. 20.
    S. Michel, M. Regnier and B. Shroot, Cornified envelope formation, an in vitro assay of retinoid activity, in: Models in Dermatology, vol. 4, H. I. Maibach and N. J. Lowe, eds., Karger, Basel (1989).Google Scholar
  21. 21.
    R. Schmidt, U. Reichert, S. Michel, B. Shroot, and M. Bouclier, Plasma membrane transglutaminase and cornified envelope competence in cultured human keratinocytes, FEBS Lett. 186:201 (1985).PubMedCrossRefGoogle Scholar
  22. 22.
    L. Fesus, V. Thomazy and A. Falus, Induction and activation of tissue transglutaminase during programmed cell death, FEBS Lett. 224:104 (1987).PubMedCrossRefGoogle Scholar
  23. 23.
    R. Marks, A. D. Pearse, T. Hashimoto and S. Barton, Overview of mode of action of retinoids, in: Retinoid Therapy. A Review of Clinical and Laboratory Research, W.J. Cunliffe and AJ. Miller, eds., MTP Press, Lancaster (1984).Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Uwe Reichert
    • 1
  • Rainer Schmidt
    • 1
  1. 1.Centre International de Recherches Dermatologiques (CIRD)ValbonneFrance

Personalised recommendations