Abstract
The skin is a peripheral organ exposed to the environment where it comes frequently in contact with toxic agents to which it may respond with irritation. This response is often the consequence of a cytotoxic action on epidermal cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
S. M. Fischer, A. Viaje, G. D. Mills and T. J. Slaga, Explant methods for epidermal cell culture, in: Methods in Cell Biology, vol. 21, C. C. Harris, B. F. Trump and G. D. Stoner, eds., Academic Press, New York (1980).
M. Pruniéras, M. Régnier and D. Woodley, Methods for cultivation of keratinocytes with an air-liquid interface, J. Invest. Dermatol. 81:28s (1983).
J. G. Rheinwald and H. Green, Serial cultivation of strains of human keratinocytes: The formation of keratinizing colonies from single cells, Cell 6:331 (1975).
S.-C. Liu, M. F. Eaton and M. A. Karasek, Growth characteristics of human epidermal keratinocytes from newborn foreskin in primary and serial culture, In Vitro 15:813 (1979).
J. Taylor-Papadimitriou, P. Purkis, E. B. Lane, I. A. McKay and S. E. Chang, Effect of SV40 transformation on the cytoskeleton and behavioural properties of human keratinocytes. Cell Differ. 11:169 (1982).
U. Reichert, Skin toxicity and cellular metabolism: in vitro models, Br. J. Dermatol. 115 (Suppl. 31):108 (1986)
U. Reichert, A. Chatelus, and G. Pautrat, Use of cultured human cells as screening models for antipsoriatic drugs, Alternative Methods in Toxicology, Vol. V, A. M. Goldberg, ed., Mary Ann Liebert, New York (1987).
U. Reichert, Y. Jacques, M. Grangeret, and R. Schmidt, Antirespiratory and antiproliferative activity of anthralin in cultured human keratinocytes, J. Invest. Dermatol. 84:130 (1985).
U. Reichert, and B. Shroot, Effect of antipsoriatic drugs on the basic metabolism of cultured human keratinocytes, in: Models in Dermatology, vol. 3, H. I. Maibach and N. J. Lowe, eds., Karger, Basel (1987).
B. Kammerau, A. Zesch and H. Schaefer, Absolute concentrations of dithranol and triacetyl-dithranol in the skin layers after local treatment: in vivo investigations with four different types of pharmacological vehicles. J. Invest. Dermatol. 64:145 (1975).
D. Cavey, R. G. Dickinson, B. Shroot and H. Schaefer, The in vivo fate of topically applied dithranol in the skin of the hairless rat, Arzneim.-Forsch. Drug Res. 35:605 (1985).
J. A. Parrish, T. B. Fitzpatrick, L. Tanenbaum and M. A. Pathak, Photochemotherapy of psoriasis with oral methoxsalen and long-wave ultraviolet light, New Engl. J. Med. 291:1207 (1974).
J. D. Laskin, E. Lee, E. J. Yurkow, D. L. Laskin and M. A. Gallo, A possible mechanism of psoralen phototoxicity not involving direct interaction with DNA. Proc. Natl. Acad. Sci. USA 82:6158 (1985).
J. D. Laskin, E. Lee, D. L. Laskin and M. A. Gallo, Psoralens potentiate ultraviolet light — induced inhibition of epidermal growth factor binding. Proc. Natl. Acad. Sci. USA 83:8211 (1986).
E. Christophers and J. Pohl, Epidermal reactions to photochemotherapy, in: The Epidermis in Disease, R. Marks and E. Christophers, eds., MTP Press, Lancaster (1981).
T. Henseler, K. Wolf, H. Hönigsmann and E. Christophers, Oral 8-methoxypsoralen photochemotherapy of psoriasis. The European PUVA study: a cooperative study among 18 European centres, Lancet, i, 853 (1981).
J. W. Melski, L. Tanenbaum, J. A. Parrish, T. B. Fitzpatrick, H. L. Bleich et al., Oral methoxsalen photochemotherapy for the treatment of psoriasis: a co-operative clinical trial, J. Invest. Dermatol. 68:328 (1977).
G. Stuettgen, Zur Lokalbehandlung von Keratosen mit Vitamin-A-Säure, Dermatologica 124:65 (1962).
B. M. Gilfix, and H. Green, Bioassay of retinoids using cultured human conjunctival keratinocytes, J. Cell. Physiol. 119:172 (1984).
S. Michel, M. Regnier and B. Shroot, Cornified envelope formation, an in vitro assay of retinoid activity, in: Models in Dermatology, vol. 4, H. I. Maibach and N. J. Lowe, eds., Karger, Basel (1989).
R. Schmidt, U. Reichert, S. Michel, B. Shroot, and M. Bouclier, Plasma membrane transglutaminase and cornified envelope competence in cultured human keratinocytes, FEBS Lett. 186:201 (1985).
L. Fesus, V. Thomazy and A. Falus, Induction and activation of tissue transglutaminase during programmed cell death, FEBS Lett. 224:104 (1987).
R. Marks, A. D. Pearse, T. Hashimoto and S. Barton, Overview of mode of action of retinoids, in: Retinoid Therapy. A Review of Clinical and Laboratory Research, W.J. Cunliffe and AJ. Miller, eds., MTP Press, Lancaster (1984).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Plenum Press, New York
About this paper
Cite this paper
Reichert, U., Schmidt, R. (1990). The Use of Cultured Human Keratinocytes as a Model System for Skin Pharmacology and Toxicology. In: Galli, C.L., Hensby, C.N., Marinovich, M. (eds) Skin Pharmacology and Toxicology. NATO ASI Series, vol 181. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7902-7_10
Download citation
DOI: https://doi.org/10.1007/978-1-4684-7902-7_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-7904-1
Online ISBN: 978-1-4684-7902-7
eBook Packages: Springer Book Archive