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Endothelium in Hemostasis and Thrombosis

  • Philip G. de Groot
  • Hetty C. de Boer
  • Pim N. M. Tijburg
  • Jaap Jan Zwaginga
Chapter
Part of the NATO ASI Series book series (volume 166)

Abstract

In the normal vasculature, blood platelets circulate freely through the vessels and no fibrin is formed. The capacity of the endothelium, which forms the inner lining of the blood vessel wall, to inhibit the activation of the coagulation cascade and to prevent platelet adhesion has been called “non-thrombogenicity”. The endothelium consists of a monolayer of cells, which have, under normal conditions, two different sites. The apical site faces the blood stream and is non-thrombogenic. On the basal site the cells synthesize an extracellular matrix, the subendothelium, which has thrombogenic properties. After contraction or desquamation of endothelial cells platelets rapidly adhere to the exposed subendothelium.

Keywords

Endothelial Cell Shear Rate Tissue Factor Platelet Adhesion Fluid Shear Stress 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Baumgartner, H.R., 1973, The role of blood flow in platelet adhesion, fibrin deposition and formation of mural thrombi, Microvasc. Res., 5: 167.PubMedCrossRefGoogle Scholar
  2. Bevilaqua, M.P., Pober, J.S., Majeau, G.R., Cotran, R.S. and Gimbrone, Jr., M.A., 1984, Interleukin-1 induces biosynthesis and cell endothelial cells, J. Exp. Med., 160: 618.CrossRefGoogle Scholar
  3. Cerveny, T., Fass, D. and Mann, K., 1984, Synthesis of factor V by cultured bovine endothelial cells, Blood, 63: 1467.PubMedGoogle Scholar
  4. Gurwen, K.D. and Gimbrone, Jr., M.A., 1980, The role of protacyclin in platelet adhesion to cultured normal and virally transformed human endothelial cells, Lab. Invest., 42: 366.Google Scholar
  5. de Groot, Ph.G., Verwey, C.L., Nawroth, P.P., de Boer, M.C., Stern, D.M. and Sixma, J.J., 1987, Interleukin-1 inhibits the synthesis of von Willebrand factor in endothelial cells which results in a decreased reactivity of their matrix towards platelets, Arteriosclerosis, 7: 605.PubMedCrossRefGoogle Scholar
  6. Dejana, E., Languino, L.R., Polentarutti, N., Balconi, G., Ryckewaert, J.J., Larrieu, M.J., Donati, M.B., Manto, Vani, A. and Margveri, G., 1985, Interaction between fibrinogen and cultured endothelial cells, J. Clin. Invest.,75:11PubMedCrossRefGoogle Scholar
  7. Dewey, Jr., C.F., Bussolari, S.R., Gimbrone, Jr., M.A. and Davies, P.F., 1981, Dynamic response of vascular cells to fluid shear stress, J. Bio. Mech. Eng., 103: 177.Google Scholar
  8. Esmon, C.T. and Owen, W.G., 1981, Identification of an endothelial protein C, Proc. Natl. Acad. Sci. USA, 78: 2249.CrossRefGoogle Scholar
  9. Fair, D.S., Marlar, R.A. and Levin, E.G., 1986, Human endothelial cells synthesize protein S., Blood, 67: 1168.PubMedGoogle Scholar
  10. Flaherty, J.T., Pierce, J.E. and Ferrans, V.J., 1972, Endothelial nuclear patterns in the canine arterial tree with particular reference to hemodynamic events, Circ. Res., 30: 23.PubMedCrossRefGoogle Scholar
  11. Frangos, J.A., Eskin, S.G., McIntire, L.V. and Ives, C.L., 1985, Flow effects on prostaglandin production by cultured human endothelial cells, Science, 227: 1477.PubMedCrossRefGoogle Scholar
  12. Gabrowski, E.F., Jaffe, E.A. and Weksler, B.B., 1985, Prostacyclin production by cultured endothelial cell monolayers exposed to step increases in shear stress, J. Lab. Clin. Med., 105: 36.Google Scholar
  13. Gimbrone, Jr., M.A., 1986, “Vascular Endothelium in Hemostasis and Trombosis”, Churchill Livingstone, Edinburgh London.Google Scholar
  14. Griffin, J.H., Evatt, B., Zimmerman, T.S., Kleis, A.J. and Widmen, C., 1981, Deficiency of protein C in congenital thrombotic disease, J. Clin. Invest., 68: 1370.PubMedCrossRefGoogle Scholar
  15. Grim, J., Keller, R. and de Groot, Ph.G., Laminar flow induces metabolism in endothelial cells, Thromb. Haemostas.,in press.Google Scholar
  16. Houdijk, W.P.M. and Sixma, J.J., 1985, Fibronectin in artery sub-endothelium is important for platelet adhesion, Blood, 65: 598.PubMedGoogle Scholar
  17. Jaffe, E.A., 1984, “Biology of endothelial cells”, Martinus Nijhoff, Boston.Google Scholar
  18. Jaffe, E.A., Hoyer, L.W. and Nachman, R.L., 1973, Synthesis of hemophilic factor antigen by cultured human endothelial cells, J. Clin. Ivest., 52: 2757.CrossRefGoogle Scholar
  19. Keller, R., Pratt, B., Furthmayer, H. and Madri, J.A., 1987, Aortic endothelial proteoheparan sulphate II: modulation by anti-extracellular matrix, Am. J. Pathol., 128: 299.PubMedGoogle Scholar
  20. Levin, E.G. and Loskutoff, D.J., 1988, Cultured bovine endothelial cells produce both urokinase and tissue type plasminogen activators, J. Cell Biol., 94: 631.CrossRefGoogle Scholar
  21. Loskutoff, D.J., van Mourik, J.A., Erickson, L.A. and Lawrence, D., 1983, Detection of an unusually stable fibrinolytic inhibitor produced by bovine endothelial cells, Proc. Natl. Acad. Sci. USA, 80: 2956.PubMedCrossRefGoogle Scholar
  22. Marcum, J.A. and Rosenberg, R.D., 1984, Anticoagulantly active heparin-like molecules from vascular tissue, Biochemistry, 23: 1730.PubMedCrossRefGoogle Scholar
  23. Mayne, R., 1986, Collagenous proteins of blood vessels. Arteriosclerosis, 6: 585.PubMedCrossRefGoogle Scholar
  24. Nawroth, P.P., Handley, D.A., Esmon, C.T. and Stern, D.M., 1986, Interleukin-1 induces endothelial cell procoagulant while suppressing cells from adult and umbilical cord arteries and veins, Thromb. Haemostas., 56: 189.Google Scholar
  25. Pearson, J.P., Carleton, J.S. and Gordon, J.L., 1980, Metabolism of adenine nucleotides by ectoenzymes on vascular endothelial and smooth muscle cells, Biochem. J., 190: 421.PubMedGoogle Scholar
  26. Prescott, S.M., Zimmerman, G.A. and McIntyre, T.M., 1984, Human endothelial cells in culture porduce platelet activating factor when stimulated with thrombin, Proc. Natl. Acad. Sci. USA, 81: 3534.PubMedCrossRefGoogle Scholar
  27. Radomski, M.W., Palmer, R.M. and Moncada, S., 1987, The role of nitric thelium, Biochem. Biophys. Res. Commun., 14: 1482.Google Scholar
  28. Reidy, M.A. and Langille, B.L., 1980, The effect of local blood flow patterns on endothelial cell morphology, Exp. Mol. Pathol., 32: 276.PubMedCrossRefGoogle Scholar
  29. Sakariassen, K.S., Aarts, P.A.M.M., de Groot, Ph.G., Houdijk, W.P.M., and Sixma,J.J., 1983, A perfusion chamber developed to investigate platelet interaction in flowing blood with human vessel wall cells, their extracellular and purified components, J. Lab. Clin. Med., 102: 522.PubMedGoogle Scholar
  30. Stern, D.M., Nawroth, P.P., Handley, D. and Kisiel, W., 1985, An endothelial cell dependent pathway of coagulation, Proc. Natl. Acad. Sci. USA, 82: 2523.PubMedCrossRefGoogle Scholar
  31. Stern, D.M., Nawroth, P.P. and Kisiel, W., 1984, A coagulation pathway on bovine aortic segments leading to generation of factor Xa and thrombin, J. Clin. Invest., 74: 1910.PubMedCrossRefGoogle Scholar
  32. Tschopp, T.B., Weiss, H.J. and Baumgartner, H.R., 1974, Decreased adhesion of platelets to subendothelium in von Willebrand disease, J. Lab. Clin. Med., 83: 296.PubMedGoogle Scholar
  33. van Hinsbergh, V.W.M., Binnema, D., Scheffer, M.A., Sprenger, E.D., Kooistra, T. and Rykeu, D.C., 1987, Production of plasminogen activators and inhibitors by serially propagated endothelial cells from adult human blood vessels, Arteriosclerosis, 7: 389.PubMedCrossRefGoogle Scholar
  34. van Wachum, P.B., Reinders, J.H., van Buul-Wortelboer, M.F., de Groot, Ph.G., van Aken, W.G., and van Mourik, J.A., 1986, Von Willebrand factor in cultured human vascular endothelial cells from adult and umbilical cord arteries and veins,Thromb. Haemostas., 56: 189.Google Scholar
  35. Weksler, B.B., Marcus, A.J. and Jaffe, E.A., 1977, Synthesis of prostaglandin I2 (prostacyclin) by cultured human and bovine endothelial cells, Proc. Natl. Acad. Sci. USA, 74: 3922.PubMedCrossRefGoogle Scholar
  36. Zwaginga, J.J., Sixma, J.J. and de Groot, Ph. G., 1987, Activation of necrosis factor or endotoxin induces platelet thrombus formation on their matrix. Studies of a new ex-vivo thrombosis model using low molecular weight heparin as anti-coagulant, Brit. J. Haematol., 66: 420.Google Scholar
  37. Zwaginga, J.J., de Boer, H.C., Kerkhof, A., Sixma, J.J. and de Groot, Ph.G., 1988, Procoagulant activity associated with matrix of cultured human vessel wall cells induces thrombus formation in an ex-vivo model using whole blood, (submitted for publication).Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Philip G. de Groot
    • 1
  • Hetty C. de Boer
    • 1
  • Pim N. M. Tijburg
    • 1
  • Jaap Jan Zwaginga
    • 1
  1. 1.Department of HaematologyUniversity Hospital UtrechtUtrechtThe Netherlands

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