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Autoradiographic Studies of DNA Replication in Werner’s Syndrome Cells

  • Fumio Hanaoka
  • Masa-atsu Yamada
  • Fujio Takeuchi
  • Makoto Goto
  • Terumasa Miyamoto
  • Tada-aki Hori
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 190)

Abstract

We have compared cultured fibroblasts of early passage derived from patients with the Werner syndrome and from normal subjects in several aspects of cell cycle time and DNA replication. The average cycle time was prolonged in Werner’s syndrome cells compared with normal cells because of changes in the duration of S phase. The durations of G1 and G2 were unchanged. In addition, the labeling index was lower in Werner’s syndrome cells, suggesting that there are two cell-cycle abnormalities in Werner’s syndrome cells. The cause of the prolongation of S phase was investigated by DNA fiber autoradiography and alkaline sucrose density gradient sedimentation. The rate of DNA chain elongation was not different in Werner’s syndrome cells from that in normal cells, but the frequency of replication initiation was decreased in Werner’s syndrome cells.

Keywords

Track Length Werner Syndrome Population Doubling Level Label Mitosis Human Diploid Cell Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Fumio Hanaoka
  • Masa-atsu Yamada
    • 1
  • Fujio Takeuchi
  • Makoto Goto
  • Terumasa Miyamoto
    • 2
  • Tada-aki Hori
    • 3
  1. 1.Department of Physiological Chemistry Faculty of Pharmaceutical SciencesUniversity of TokyoJapan
  2. 2.Department of Internal Medicine and Physical Therapy Faculty of MedicineUniversity of TokyoJapan
  3. 3.Division of GeneticsNational Institute of Radiological SciencesChibaJapan

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