Growth Characteristics of Werner Syndrome Cells in Vitro

  • Darrell Salk
  • Eileen Bryant
  • Holger Hoehn
  • Patricia Johnston
  • George M. Martin
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 190)

Abstract

Cultured skin fibroblast-like (FL) cells from patients with Werner syndrome grow poorly: reduced growth potential has been reported by more than 11 laboratories for cultures from more than 26 patients, using many different tissue culture media. This characteristic is of importance to research in Werner syndrome for several reasons. It remains to be determined whether poor growth is a primary or a secondary manifestation of the basic molecular defect, and the relationship is not yet clear between reduced growth of cultured skin fibroblasts and the other manifestations of Werner syndrome in vitro and in vivo. This characteristic also makes it difficult to obtain sufficient growth for clonal studies or for biochemical studies that require a large number of cells.

Keywords

Fibril Thymidine 

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References

  1. Matsumura, T., Zerrudo, Z., Hayflick, L. (1979). Senescent human diploid cells in culture: Survival, DNA synthesis and morphology. J. Gerontol. 34: 328–334.Google Scholar
  2. Rabinovitch, P. S., Norwood, T. H. (1980). Comparative heterokaryon study of cellular senescence and the serum-deprived state. Exp. Cell Res. 130: 101–109.Google Scholar
  3. Salk, D., Bryant, E., Hoehn, H., Martin, G. M. (1981), Systematic growth studies, cocultivation, and cell hybridization studies of Werner syndrome cultured skin fibroblasts. Hum. Genet. 58: 310–316.Google Scholar
  4. Salk, D. (1982). Werner’s syndrome: A review of recent research with an analysis of connective tissue metabolism, growth control of cultured cells, and chromosomal aberrations.um. Genet. 62: 1–15.Google Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Darrell Salk
    • 1
  • Eileen Bryant
    • 1
  • Holger Hoehn
    • 2
  • Patricia Johnston
    • 1
  • George M. Martin
    • 1
  1. 1.Department of PathologyUniversity of WashingtonUSA
  2. 2.Department of Human GeneticsUniversity of WuerzburgGermany

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