Structural Fingerprinting of Gangliosides and Other Glycoconjugates by Mass Spectrometry

  • Karl-Anders Karlsson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 125)

Abstract

In view of the already long use of permethylation for structural studies of carbohydrate it is surprising that the first mass spectra of non-degraded permethylated derivatives of glycolipids appeared rather late (KARLSSON, 1973). Since then several laboratories have adopted this technique as an important supplement to conventional degradation methods. However, only one other research group (see WATANABE et al., 1978) has added the use of permethylated-reduced derivatives (see below), which we consider necessary for a safe conclusion. One purpose of the present communication is to illustrate the importance of a combined use of the two derivatives. Secondly, the potent application on mixtures of glycolipid antigens will be shown. In this case mass spectrometry is the only microchemical method available today able of a specific detection of separate sequences in a mixture.

Keywords

Sugar Saccharide Amide Polysaccharide Oligosaccharide 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. BREIMER, M.E., HANSSON, G.C., KARLSSON, K.-A., LEFFLER, H., PIMLOTT, W. and SAMUELSSON, B.E. (1978): Structure determination of blood group type glycolipids of cat small intestine by mass fragmentography. FEBS Lett. 89, 42–46.PubMedCrossRefGoogle Scholar
  2. BREIMER, M.E., HANSSON, G.C., KARLSSON, K.-A., LEFFLER, H., PIMLOTT, W. and SAMUELSSON, B.E. (1979): Selected ion monitoring of glycosphingolipid mixtures. Identification of several blood group type glycolipids in the small intestine of an individual rabbit. Biomed. Mass Spectrom. 6, in press.Google Scholar
  3. HAKOMORI, S.-I. (1964): A rapid permethylation of glycolipid and polysaccharide, catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide. J. Biochem. 53, 205–208.Google Scholar
  4. KARLSSON, K.-A. (1973): Carbohydrate composition and sequence analysis of cell surface components by mass spectrometry. Characterization of the major monosialoganglioside of brain. FEBS Lett. 32, 317–320.Google Scholar
  5. KARLSSON, K.-A. (1974): Carbohydrate composition and sequence analysis of a derivative of brain disialoganglioside by mass spectrometry, with molecular weight ions at m/e 2245. Potential use in the specific microanalysis of cell surface components. Biochemistry 13, 3643–3647.Google Scholar
  6. KARLSSON, K.-A. (1976): Microscale fingerprinting of blood-group fucolipids by mass spectrometry, in “Glycolipid Methodology”, Witting, L.A. ed., American Oil Chemists’ Society ( Champaign, Illinois ), pp. 97–122.Google Scholar
  7. KARLSSON, K.-A. (1977): Mass-spectrometric sequence studies of lipid-linked oligosaccharides. Blood-group fucolipids, ganglio-sides and related cell-surface receptors. Progr. Chem. Fats Other Lipids 16, 207–230.Google Scholar
  8. KARLSSON, K.-A., PASCHER, I. and SAMUELSSON, B.E. (1974): Analysis of intact gangliosides by mass spectrometry. Comparison of different derivatives of a hematoside of a tumor and the major monosialoganglioside of brain. Chem. Phys. Lipids 12, 271–286.Google Scholar
  9. KARLSSON, K.-A., PASCHER, I., SAMUELSSON, B.E., FINNE, J., KRUSIUS, T. and RAUVALA, H. (1978): Mass spectrometric sequence study of the oligosaccharide of human transferrin. FEBS Lett. 94, 413–417.PubMedCrossRefGoogle Scholar
  10. WATANABE, K., POWELL, M. and HAKOMORI, S.-I. (1978): Isolation and characterization of a novel fucoganglioside of human erythrocyte membranes. J. Biol. Chem. 253, 8962–8967.Google Scholar
  11. WIEGANDT, H., ZIEGLER, W., STAERK, J., KRANZ, T., RONNEBERGER, H.J., ZILG, H., KARLSSON, K.-A. and SAMUELSSON, B.E. (1976): Studies of the ligand binding to cholera toxin. I. The lipophilic moiety of sialoglycolipids. Hoppe-Seyler’s Z. Physiol. Chem. 357, 1637–1646.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Karl-Anders Karlsson
    • 1
  1. 1.Department of Medical BiochemistryUniversity of GöteborgGöteborgSweden

Personalised recommendations