Structural Fingerprinting of Gangliosides and Other Glycoconjugates by Mass Spectrometry

  • Karl-Anders Karlsson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 125)


In view of the already long use of permethylation for structural studies of carbohydrate it is surprising that the first mass spectra of non-degraded permethylated derivatives of glycolipids appeared rather late (KARLSSON, 1973). Since then several laboratories have adopted this technique as an important supplement to conventional degradation methods. However, only one other research group (see WATANABE et al., 1978) has added the use of permethylated-reduced derivatives (see below), which we consider necessary for a safe conclusion. One purpose of the present communication is to illustrate the importance of a combined use of the two derivatives. Secondly, the potent application on mixtures of glycolipid antigens will be shown. In this case mass spectrometry is the only microchemical method available today able of a specific detection of separate sequences in a mixture.


Sialic Acid Cholera Toxin Human Transferrin Neuraminic Acid High Mass Region 
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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Karl-Anders Karlsson
    • 1
  1. 1.Department of Medical BiochemistryUniversity of GöteborgGöteborgSweden

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