Advertisement

Thyrotropin Receptors and Gangliosides

  • Leonard D. Kohn
  • Eduardo Consiglio
  • Marc J. S. Dewolf
  • Evelyn F. Grollman
  • Fred D. Ledley
  • George Lee
  • Nicholas P. Morris
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 125)

Abstract

Recent studies (1, 2, 28–30, 33–35) have implicated ganglio-sides in the receptor recognition process for thyrotropin (TSH) and have led to the hypothesis (15–17, 19) that the TSH receptor is structurally and functionally related to receptors for other ligands which interact with gangliosides in vitro, i.e., cholera toxin, tetanus toxin, and interferon. This report summarizes the accumulated evidence which implicates both a glycoprotein (4, 38–40, 43) and a ganglioside in the structure and function of the TSH receptor. In the context of this symposium, particular attention will be addressed to the role of the ganglioside.

Keywords

Cholera Toxin Thyroid Cell Tetanus Toxin Glycoprotein Hormone Thyrotropin Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Bibliography

  1. 1.
    ALOJ S.M., KOHN L.D., LEE G. and MELDOLESI M.G. (1977): The binding of thyrotropin to liposomes containing ganglio-sides. Biochem. Biophys. Res. Commun. 74, 1053–1059.Google Scholar
  2. 2.
    ALOJ S.M., LEE G., CONSIGLIO E., FORMISANO S., MINTON A.P. and KOHN L.D. (1979): Dansylated thyrotropin as a probe of hormone receptor interactions. J. Biol. Chem. 254, in press.Google Scholar
  3. 3.
    ALOJ S.M., LEE G., GROLLMAN E.F., BEGUINOT F., CONSIGLIO E. and KOHN L.D. (1979): Role of phospholipids in the structure and function of the thyrotropin receptor. J. Biol. Chem. 254, in press.Google Scholar
  4. 4.
    AMIR S.M., CARRAWAY T.F. Jr., KOHN L.D. and WINAND R.J. (1972): Thyrotropin binding to thyroid plasma membranes. J. Biol. Chem. 248, 4092–4100.Google Scholar
  5. 5.
    BENNETT V. and CUATRECASAS P. (1977): Membrane gangliosides and activation of adenylate cyclase, in “The Specificity and Action of Animal, Bacterial, and Plant Toxins”, CUATRECASAS P. and GREAVES M.F., Eds., Chapman and Hall (London), pp. 3–66.Google Scholar
  6. 6.
    CHANG E.H., GROLLMAN E.F., JAY F.T., LEE G., KOHN L.D. and FRIEDMAN R.M. (1978): Membrane alterations following interferon treatment, in “Human Interferon”, STINEBRING W.R. and CHAPPLE P.J., Eds., Plenum (New York), pp. 85–99.Google Scholar
  7. 7.
    FORMISANO S., LEE G., JOHNSON M.L., and EDELHOCH H. (1979): Critical micelle concentrations of gangliosides. Biochemistry 18, in press.Google Scholar
  8. 8.
    FRIEDMAN R.M. and KOHN L.D. (1976): Cholera toxin inhibits interferon action. Biochem. Biophys. Res. Commun. 70, 1078–1084.Google Scholar
  9. 9.
    GROLLMAN E.F., LEE G., AMBESI-IMPIOMBATO F.D., MELDOLESI M.F., ALOJ S.M., COON H.G., KABACK H.R. and KOHN L.D. (1977): Effects of thyrotropin on the thyroid cell membrane: hyper-polarization induced by hormone-receptor interaction. Proc. Natl. Acad. Sci. U.S.A. 74, 2352–2356.Google Scholar
  10. 10.
    GROLLMAN E.F., LEE G., RAMOS S., LAZO P.S., KABACK H.R., FRIEDMAN R.M. and KOHN L.D. (1978): Relationships of the structure and function of the interferon receptor to hormone receptors and establishment of the antiviral state. Cancer Res. 38, 4172–4185.PubMedGoogle Scholar
  11. 11.
    HABIG W.H., GROLLMAN E.F., LEDLEY F.D., MELDOLESI M.F., ALOJ S.M., HARDEGREE M.C. and KOHN L.D. (1978): Tetanus toxin interactions with the thyroid: decreased toxin binding to membranes from a thyroid tumor with a thyrotropin receptor defect and in Vivostimulation of thyroid function. Endocrinology 102, 844–851.PubMedCrossRefGoogle Scholar
  12. 12.
    HABIG W.H., HARDEGREE M.C., GROLLMAN E.F., LEE G., RAMOS S. KOHN L.D. (1979): Interaction of tetanus toxin with thyroid membranes, in Proceedings of the 5th International Tetanus Conference, Stockholm, Sweden, June 18–23, 1978, in press.Google Scholar
  13. 13.
    HILL I.R., LEVIN I.W., LAZO P.S. KOHN L.D. (1979): Effect of ganglioside and ganglioside-cholera toxin interactions on the vibrational Raman spectra of the bilayer lipid in dipalmitoyl phosphatidylcholine liposomes. Biochemistry 18, in press.Google Scholar
  14. 14.
    JAQUES L.W., BROWN E.B., BARRETT J.M., BREY W.S. and WELLNER W. (1977): Sialic acid: a calcium binding carbohydrate. J. Biol. Chem. 252, 4533–4538.PubMedGoogle Scholar
  15. 15.
    KOHN L.D. (1977): Characterization of the thyrotropin receptor and its involvement in Graves’ disease, in “Horizons in Biochemistry and Biophysics”, QUAGLIARIELLO E., Ed., Vol. 3, Addison-Wesley (Reading, Massachusetts ), pp. 123–163.Google Scholar
  16. 16.
    KOHN L.D. (1977): Relationships in the structure and function of cell surface receptors for glycoprotein hormones, bacterial toxins, and interferon, in “Annual Reports in Medicinal Chemistry”, CLARKE F.H., Ed., Vol. 12, Academic Press (New York), pp. 211–222.Google Scholar
  17. 17.
    KOHN L.D. (1978): Relationships in the structure and function of receptors for glycoprotein hormones, bacterial toxins, and interferon, in “Receptors and Recognition”, CUATRECASAS P. and GREAVES M.F., Eds., Series A, Vol. 5, Chapman and Hall (London), pp. 134–212.Google Scholar
  18. 18.
    KOHN L.D., FRIEDMAN R.M., HOLMES J.M. and LEE G. (1976): Use of thytrotopin and cholera toxin to probe the mechanism by which interferon initiates its antiviral activity. Proc. Natl. Acad. Sci. U.S.A. 73, 3695–3699.PubMedCrossRefGoogle Scholar
  19. 19.
    KOHN L.D., LEE G., GROLLMAN E.F., LEDLEY F.D., MULLIN B.R., FRIEDMAN R.M., MELDOLESI M.F. and ALOJ S.M. (1978): Membrane glycolipids and their relationship to the structure and function of cell surface receptors for glycoprotein hormones, bacterial toxins, and interferon, in “Cell Surface Carbohydrate Chemistry”, HARMAN R.E. ( Ed. ), Academic Press (New York), pp. 103–133.Google Scholar
  20. 20.
    KUROSKY A., MARKEL D.E., PETERSON J.W. and FITCH W.M. (1977): Primary structure of cholera toxin 13-chain: a glycoprotein hormone analog? Science 195, 299–301.PubMedCrossRefGoogle Scholar
  21. 21.
    LAZO P.S., GROLLMAN E.F., LEE G. and FRIEDMAN R.M. (1978): Structure:function studies of the interferon receptor. Fed. Proc. 37, 1822.Google Scholar
  22. 22.
    LEDLEY F.D., LEE G., KOHN L.D., HABIG W.H. and HARDEGREE M.C. (1977): Tetanus toxin interactions with thyroid plasma membranes: implications for structure and function of tetanus toxin receptors and potential pathophysiological significance. J. Biol. Chem. 252, 4049–4055.PubMedGoogle Scholar
  23. 23.
    LEDLEY F.D., MULLIN B.R., LEE G., ALOJ S.M., FISHMAN P.H., HUNT L.T., DAYHOFF M.O. and KOHN L.D. (1976): Sequence similarity between cholera toxin and glycoprotein hormones: implications for structure activity relationship and mechanism of action. Biochem. Biophys. Res. Commun. 69, 852–859.Google Scholar
  24. 24.
    LEE G., ALOJ S.M., BRADY R.O. and KOHN L.D. (1976): The structure and function of glycoprotein hormone receptors: ganglioside interactions with human chorionic gonadotropin. Biochem. Biophys. Res. Commun. 73, 370–377.Google Scholar
  25. 25.
    LEE G., ALOJ S.M. and KOHN L.D. (1977): The structure and function of glycoprotein hormone receptors: ganglioside interactions with luteinizing hormone. Biochem. Biophys. Res. Commun. 77, 434–441.Google Scholar
  26. 26.
    LEE G., CONSIGLIO E., HABIG W.H., DYER S., HARDEGREE M.C. and KOHN L.D. (1978): Structure:function studies of receptors for thyrotropin and tetanus toxin: lipid modulation of effector binding to the glycoprotein receptor component. Biochem. Biophys. Res. Commun. 83, 313–320.Google Scholar
  27. 27.
    LEE G., CONSIGLIO E., HABIG W.H., DYER S., HARDEGREE M.C. and KOHN L.D. (1978): Structure:function studies of receptors for thyrotropin and tetanus toxin: lipid modulation of effector binding to the glycoprotein receptor component. Biochem. Biophys. Res. Commun. 83, 313–320.Google Scholar
  28. 28.
    LEE G., GROLLMAN E.F., ALOJ S.M., KOHN L.D. and WINAND R.J. (1977): Abnormal adenylate cyclase activity and altered gangliosides in thyroid cells from patients with Graves’ disease. Biochem. Biophys. Res. Commun. 77, 139–146.Google Scholar
  29. 29.
    MELDOLESI M.F., FISHMAN P.H., ALOJ S.M., KOHN L.D. and BRADY R.O. (1976): Relationship of gangliosides to the structure and function of thyrotropin receptors: their absence on plasma membranes of a thyroid tumor defective in thyrotropin activity. Proc. Natl. Acad. Sci. U.S.A. 73, 4060–4064.CrossRefGoogle Scholar
  30. 30.
    MELDOLESI M.F., FISHMAN P.H., ALOJ S.M., LEDLEY F.D., LEE G., BRADLEY R.M., BRADY R.O. and KOHN L.D. (1977): Separation of the glycoprotein and ganglioside components of thyrotropin receptor activity in plasma membranes. Biochem. Biophys. Res. Commun. 75, 581–587.Google Scholar
  31. 31.
    MOSS J., FISHMAN P.H., RICHARDS R.L., ALVING C.R., VAUGHAN M. and BRADY R.O. (1976): Choleragen-mediated release of trapped glucose from liposomes containing ganglioside GM1• Proc. Natl. Acad. Sci. U.S.A. 73, 3480–3483.CrossRefGoogle Scholar
  32. 33.
    MOSS J MANGANIELLO V.C. and VAUGHAN M. (1977): Hydrolysis of nicotinamide adenine dinucleotide by choleragen and its A protomer: possible role in the activation of adenylate cyclase. Proc. Natl. Acad. Sci. U.S.A. 73, 4424–4427.Google Scholar
  33. 33.
    MULLIN B.R., ALOJ S.M., FISHMAN P.H., LEE G., KOHN L.D. and BRADY R.O. (1976): Cholera toxin interactions with thyrotropin receptors on thyroid plasma membranes. Proc. Natl. Acad. Sci. U.S.A. 73, 1679–1683.PubMedCrossRefGoogle Scholar
  34. 34.
    MULLIN B.R., FISHMAN P.H., LEE G., ALOJ S.M., LEDLEY F.D., WINAND R.J., KOHN L.D. and BRADY R.O. (1976): Thyrotropinganglioside interactions and their relationship to the structure and function of thyrotropin receptors. Proc. Natl. Acad. Sci. U.S.A. 73, 842–846.PubMedCrossRefGoogle Scholar
  35. 35.
    MULLIN B.R., PACUSZKA T., LEE G., KOHN L.D., BRADY R.O. and FISHMAN P.H. (1978): Thyroid gangliosides with high affinity for thyrotropin: potential role in thyroid regulation. Science 199, 77–79.PubMedCrossRefGoogle Scholar
  36. 36.
    POSS A., DELEERS M. and RUJSSCHERT J.M. (1978): Evidence for a specific interaction between GT1 ganglioside incorporated into bilayer membranes and thyrotropin binding. FEBS Letts. 86, 160–162.CrossRefGoogle Scholar
  37. 37.
    POSS A., DELEERS M. and RUJSSCHERT J.M. (1978): Evidence for a specific interaction between GT1 ganglioside incorporated into bilayer membranes and thyrotropin binding. FEBS Letts. 86, 160–162.CrossRefGoogle Scholar
  38. 38.
    TATE R.L., HOLMES J.M., KOHN L.D. and WINAND R.J. (1975): Characteristics of a solubilized thyrotropin receptor from bovine thyroid plasma membranes. J. Biol. Chem. 250, 6527–6533.Google Scholar
  39. 39.
    TATE R.L., SCHWARTZ H.I., HOLMES J.M., KOHN L.D. and WINAND R.J. (1975): Thyrotropin receptors in thyroid plasma membranes. J. Biol. Chem. 250, 6509–6515.PubMedGoogle Scholar
  40. 40.
    TATE R.L., WINAND R.J. and KOHN L.D. (1976): Solubilization and partial purification of the thyrotropin receptor, in “Thyroid Research”, ROBBINS J. and BRAVERMAN L., Eds., International Congress Series No. 378, Excerpta Medica (Amsterdam), pp. 57–60.Google Scholar
  41. 41.
    TOSTESON M.T. and TOSTESON D.C. (1978): Bilayers containing gangliosides develop channels when exposed to cholera toxin. Nature 275, 142–144.PubMedCrossRefGoogle Scholar
  42. 3429.
    WADELEUX P.A., ETIENNE-DECERF J., WINAND R.J. and KOHN L.D. (1978): Effects of thyrotropin on iodine metabolism of dog thyroid cells in tissue culture. Endocrinology 102, 889–902.Google Scholar
  43. 43.
    WINAND R.J. and KOHN L.D. (1975): Thyrotropin effects on thyroid cells in culture: effects of trypsin on the thyrotropin-medicated cyclic 3’:5’-AMP changes. J. Biol. Chem. 250, 6534–6540.PubMedGoogle Scholar
  44. 44.
    YOHE H.C., ROARK D.E. and ROSENBERG A. (1976): C20-Sphingosine as a determining factor in aggregation of gangliosides. J. Biol. Chem. 251, 7083–7087.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Leonard D. Kohn
    • 1
  • Eduardo Consiglio
    • 1
    • 2
  • Marc J. S. Dewolf
    • 1
    • 3
  • Evelyn F. Grollman
    • 1
  • Fred D. Ledley
    • 1
  • George Lee
    • 1
  • Nicholas P. Morris
    • 1
  1. 1.Section on Biochemistry of Cell Regulation, Laboratory of Biochemical Pharmacology, National Institute of Arthritis, Metabolism, and Digestive DiseasesNational Institutes of HealthBethesdaUSA
  2. 2.Centro di Endocrinologia ed Oncologia Sperimentale del C.N.R.NaplesItaly
  3. 3.RUCA-Laboratory for Human BiochemistryUniversity of AntwerpAntwerpBelgium

Personalised recommendations