A new anion exchange resin, Spherosil-DEAE-Dextran, built on porous glass beads covered with crosslinked DEAE-Dextran, has been compared with other resins, DEAE-Sephacel, DEAE-Sephadex and DEAE-Sepharose, regarding its value in the isolation and separation of gangliosides from a crude ganglioside extract. In comparison with the best commercial resin, DEAE-Sepharose, Spherosil-DEAE-Dextran had higher binding capacity, higher binding specificity and gave a better separation of gangliosides with four and five sialic acids. The constant bed volume facilitated regeneration.
Gangliosides were separated according to the number of sialic acids by stepwise elution with potassium acetate in methanol. The elution scheme was used for both large and small scale separation.
Separation of gangliosides on anion exchange resins according to their number of sialic acids results in a better discrimination and identification of the many closely allied gangliosides. This property is particularly important in the separation of gangliosides with a wide variation in the substitution of the sialic acid, N-acetyl, N-glycolyl or N,O-diacetyl groups. An N,O-diacetyl sialic acid will exhibit chemical properties very similar to those of a ganglioside with the N-acetyl sialic acid in lacton form, but they could be separated on the anion exchanger.
Sialic Acid Anion Exchange Resin Potassium Acetate Lacton Form High Binding Capacity
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