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Immunoassays Based on Plastic-Adsorbed Gangliosides

  • J. Holmgren
  • H. Elwing
  • P. Fredman
  • L. Svennerholm
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 125)

Abstract

Important qualities of good immunoassays include high specificity, sensitivity, accuracy and simplicity in performance. These criteria are only met to a limited extent by the traditional immunological methods registering e.g. precipitation, agglutination or complement fixation. Therefore, in recent years these methods have gradually been replaced by various “primary binding” assays. These methods often use insolubilized antigen (or antibody) as a means to enable effective separation of bound and unbound reagents and a radioisotope- or enzyme-label for the detection step.

Keywords

Cholera Toxin Plastic Surface Ganglioside Conc Crude Toxin Test Ligand 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. ELWING H., NILSSON L.-A. and OUCHTERLONY Ö. (1976): Visualization principles in thin-layer immunoassays (TIA) on plastic surfaces. Int. Archs. Allergy appl. Immun. 51, 757–762.Google Scholar
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  3. HOLMGREN J. and SVENNERHOLM A.-M. (1973): Enzyme--linked immunosorbent assays for cholera serology. Infect. Immun. 5, 662–667.Google Scholar
  4. SVENNERHOLM A.-M. and HOLMGREN J. (1978): Identification of Escherichia coli heat-labile enterotoxin by means of a ganglioside immunosorbent assay (GM1-ELISA) procedure. Curr. Microbiol. 1, 19–27.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • J. Holmgren
    • 1
  • H. Elwing
    • 1
  • P. Fredman
    • 2
  • L. Svennerholm
    • 2
  1. 1.Institute of Medical MicrobiologyUniversity of GöteborgGöteborgSweden
  2. 2.Department of NeurochemistryUniversity of GöteborgGöteborgSweden

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