Immunoassays Based on Plastic-Adsorbed Gangliosides
Important qualities of good immunoassays include high specificity, sensitivity, accuracy and simplicity in performance. These criteria are only met to a limited extent by the traditional immunological methods registering e.g. precipitation, agglutination or complement fixation. Therefore, in recent years these methods have gradually been replaced by various “primary binding” assays. These methods often use insolubilized antigen (or antibody) as a means to enable effective separation of bound and unbound reagents and a radioisotope- or enzyme-label for the detection step.
KeywordsCholera Toxin Plastic Surface Ganglioside Conc Crude Toxin Test Ligand
Unable to display preview. Download preview PDF.
- ELWING H., NILSSON L.-A. and OUCHTERLONY Ö. (1976): Visualization principles in thin-layer immunoassays (TIA) on plastic surfaces. Int. Archs. Allergy appl. Immun. 51, 757–762.Google Scholar
- HOLMGREN J. and LÖNNROTH I. (1979): Structure and function of enterotoxins and their receptors. In “43rd Nobel Symposium. Cholera and Related Diarrheas. Molecular aspects on a global health problem”, eds. OUCHTERLONY Ö and HOLMGREN J., Karger, Basel.Google Scholar
- HOLMGREN J. and SVENNERHOLM A.-M. (1973): Enzyme--linked immunosorbent assays for cholera serology. Infect. Immun. 5, 662–667.Google Scholar