Skip to main content

A Sensitive Assay for Gangliosides in the Subnanomole Range

  • Chapter
Structure and Function of Gangliosides

Abstract

The analysis of the chemical quantity of glycosphingolipids during ontogenic development or transformation of cells is as important as the characterization of their glycosphingolipid pattern and metabolism.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • COLES, L. and GRAY, G.M. (1970): Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids. J. Lipid. Res. 11, 164–166

    PubMed  CAS  Google Scholar 

  • FOLCH, J., LEES, M. and SLOANE-STANLEY, G.H. (1957): A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509

    PubMed  CAS  Google Scholar 

  • GAVER, R.C. and SWEELEY, C.C. (1965): Methods for methanolysis of sphingolipids and direct determination of long-chain bases by gas chromatography. J. Am. Oil Chemists’ Soc. 42, 294–298

    Article  CAS  Google Scholar 

  • KISIC, A. and RAPPORT, M.M. (1974): Determination of long-chain base in glycosphingolipids with Fluorescamine. J. Lipid Res. 15, 179–180

    PubMed  CAS  Google Scholar 

  • NAOI, M., LEE, Y.C. and ROSEMAN, S. (1974): Rapid and sensitive determination of sphingosine bases and sphingolipids with Fluorescamine. Anal. Biochem. 58, 571–577

    Article  PubMed  CAS  Google Scholar 

  • OULEVEY, J., BODDEN, E. and THIELE, O.W. (1977): Quantitative determination of glycosphingolipids illustrated by using erythrocyte membranes of various mammalian species. Eur. J. Biochem. 79, 265–267

    Article  PubMed  CAS  Google Scholar 

  • SAITO, T. and HAKOMORI, S.-I. (1971): Quantitative isolation of total glycosphingolipids from animal cells. J. Lipid Res. 12, 257–259

    PubMed  CAS  Google Scholar 

  • SCHWARZMANN, G. (1978): A simple and novel method for tritium labeling of gangliosides and other sphingolipids. Biochim. Biophys. Acta 529, 106–114

    Article  PubMed  CAS  Google Scholar 

  • SVENNERHOLM, L. (1957): Quantitative estimation of sialic acids. Biochim. Biophys. Acta 24, 604–611

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1980 Plenum Press, New York

About this chapter

Cite this chapter

Schwarzmann, G., Schlemmer, U., Wiegandt, H. (1980). A Sensitive Assay for Gangliosides in the Subnanomole Range. In: Svennerholm, L., Mandel, P., Dreyfus, H., Urban, PF. (eds) Structure and Function of Gangliosides. Advances in Experimental Medicine and Biology, vol 125. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7844-0_19

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-7844-0_19

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-7846-4

  • Online ISBN: 978-1-4684-7844-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics