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Brain and Retinal Ganglioside Composition from Different Species Determined by TLC and HPTLC

  • P. F. Urban
  • S. Harth
  • L. Freysz
  • H. Dreyfus
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 125)

Abstract

Gangliosides are compounds found in the CNS at relatively high concentrations (MORGAN et al., 1971, 1973; TETTAMANTI et al., 1972; BRECKENRIDGE, GOMBOS and MORGAN, 1972). In establishing the physiological role of gangliosides, their distribution in different brain areas, cell types, and subcellular fractions should be useful. The presence of a high amount of gangliosides in plasma membranes and in synaptic junctions has been established (HAMBERGER and SVENNERHOLM, 1971; WIEGANDT, 1967).

Keywords

Total Lipid Extract Rabbit Brain Chick Brain Chick Retina Synaptosomal Plasma Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. ANDO S., CHANG N. and YU R.K. (1978): High-performance thin-layer chromatography and densitometric determination of brain ganglioside compositions of several species. Anal. Biochem. 89, 437–450.Google Scholar
  2. BRECKENRIDGE W.C., GOMBOS G. and MORGAN I.G. (1972): The lipid composition of adult rat brain synaptosomal plasma membranes. Biochim. Biophys. Acta 266, 695–707.PubMedCrossRefGoogle Scholar
  3. COCHRAN F.B., YU R.K. and LEDEEN R.W. (1977): Comparison of CNS myelin gangliosides in several vertebrate species. 6th Intern. Meeting of Neurochemistry, Copenhagen, Denmark, Vol. 6, Abstr. p. 540, n° 496.Google Scholar
  4. DREYFUS H., URBAN P.F., EDEL-HARTH S. and MANDEL P. (1975): Developmental patterns of gangliosides and of phospholipids in chick retina and brain. J. Neurochem. 25, 245–250.PubMedCrossRefGoogle Scholar
  5. DREYFUS H., URBAN P.F., HARTH S., PRETI A. and MANDEL P. (1976): Retinal gangliosides: composition, evolution with age. Biosynthetic and metabolic approaches, in “Ganglioside Function”, PORCELLATI G., CECCARELLI B. and TETTAMANTI G., Eds., Advances in Experimental Biology, Vol. 71, Plenum Press (New York and London), pp. 163–188.Google Scholar
  6. FEWSTER M.E., BLACKSLONE S.C. and IHRIQ T.Y. (1973): The preparation and characterization of isolated oligodendroglia from bovine white matter. Brain Res. 63, 263–271.PubMedCrossRefGoogle Scholar
  7. FREYSZ L., FAROOQUI A.A., ADAMCZEWSKA-GONCERZEWICZ Z. and MANDEL P. (1979): Lysosomal hydrolases in neuronal, astroglial and oligodendroglial enriched fractions of rabbit and beef brain. J. Lipid Res. 20, 503–508.PubMedGoogle Scholar
  8. HAMBERGER A. and SVENNERHOLM L. (1971): Composition of gangliosides and phospholipids of neuronal and glial cell enriched fractions. J. Neurochem. 18, 1821–1829.PubMedCrossRefGoogle Scholar
  9. HARTH S., DREYFUS H., URBAN P.F. and MANDEL P. (1978): Direct thin layer chromatography of gangliosides of a total lipid extract. Anal. Biochem. 86, 543–551.PubMedCrossRefGoogle Scholar
  10. IQBAL K. and TELLEZ-NAGEL I. (1972): Isolation of neurons and glial cells from normal and pathological human brains. Brain Res. 45, 296–301.PubMedCrossRefGoogle Scholar
  11. LEDEEN R.W., YU R.K. and ENG L.F. (1973): Gangliosides of human myelin: sialosyl-galactosylceramide (G7) as a major component. J. Neurochem. 21, 829–839.PubMedCrossRefGoogle Scholar
  12. MORGAN I.G., ZANETTA J.P., BRECKENRIDGE W.C., VINCENDON G. and GOMBOS G. (1973): The chemical structure of synaptic membranes. Brain Res. 62, 405–411.PubMedCrossRefGoogle Scholar
  13. MORGAN I.G., WOLFE L.S., MANDEL P. and GOMBOS G. (1971): Isolation of plasma membranes from rat brain. Biochim. Biophys. Acta 241, 737–751.PubMedCrossRefGoogle Scholar
  14. PODUSLO S.E. and NORTON W.T. (1975): Isolation of specific brain cells. Methods in Enzymol. 35, 561–576.CrossRefGoogle Scholar
  15. SUZUKI K. (1964): A simple and accurate micromethod for quantitative determination of ganglioside patterns. Life Sci. 3, 1227–1233.PubMedCrossRefGoogle Scholar
  16. SVENNERHOLM L. (1963): Chromatographic separation of human brain gangliosides. J. Neurochem. 10, 613–623.PubMedCrossRefGoogle Scholar
  17. TETTAMANTI G., MORGAN I.G., GOMBOS G., VINCENDON G. and MANDEL P. (1972): Sub-synaptosomal localization of brain particulate neuraminidase. Brain Res. 47, 515–518.PubMedCrossRefGoogle Scholar
  18. WIEGANDT H. (1967): The subcellular localization of gangliosides in the brain. J. Neurochem. 14, 671–674.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • P. F. Urban
    • 1
    • 2
  • S. Harth
    • 1
    • 2
  • L. Freysz
    • 1
    • 2
  • H. Dreyfus
    • 1
    • 3
  1. 1.Unité 44 de l’INSERMCentre de Neurochimie du CNRSStrasbourg CedexFrance
  2. 2.Chargés de Recherche au CNRSFrance
  3. 3.Chargé de Recherche à l’INSERMFrance

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