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Enzymic Degradation of Gangliosides

  • Yu-Teh Li
  • May-Jean King
  • Su-Chen Li
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 125)

Abstract

As in the case for the enzymic degradation of sugar chains in other glycoconjugates, the following considerations should be made in investigating the enzymic degradation of saccharide chains in gangliosides. One should be aware of the fact that the same glycosidase isolated from different sources may vary considerably in its substrate specificity. In some instances, the reactions are greatly affected by the amount and the nature of the detergent or the activator to be included in the reaction mixture. In addition, one should consider the substrate concentration and the ionic strength of the buffer, as they may severely affect enzyme activity. It is also very important to realize that O-acetylated or O-methylated glycons are often resistant to glycosidases. Finally, one should bear in mind that the glycosidases used may be contaminated with one or several unexpected enzyme activities.

Keywords

Ionic Strength Enzymic Hydrolysis Sialic Acid Enzymic Degradation Buffer Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Yu-Teh Li
    • 1
  • May-Jean King
    • 1
  • Su-Chen Li
    • 1
  1. 1.Department of BiochemistryTulane University School of Medicine and Delta Primate CenterNew OrleansUSA

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