Enzymic Degradation of Gangliosides
As in the case for the enzymic degradation of sugar chains in other glycoconjugates, the following considerations should be made in investigating the enzymic degradation of saccharide chains in gangliosides. One should be aware of the fact that the same glycosidase isolated from different sources may vary considerably in its substrate specificity. In some instances, the reactions are greatly affected by the amount and the nature of the detergent or the activator to be included in the reaction mixture. In addition, one should consider the substrate concentration and the ionic strength of the buffer, as they may severely affect enzyme activity. It is also very important to realize that O-acetylated or O-methylated glycons are often resistant to glycosidases. Finally, one should bear in mind that the glycosidases used may be contaminated with one or several unexpected enzyme activities.
KeywordsIonic Strength Enzymic Hydrolysis Sialic Acid Enzymic Degradation Buffer Concentration
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