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Kinetics of the Phagocytosis of Repeated Injections of Colloidal Carbon: Blockade, a Latent Period or Stimulation? A Question of Timing and Dose

  • Ernest L. Dobson
  • Lola S. Kelly
  • Caroline R. Finney
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1)

Abstract

The phenomenon of “blockade” has been attributed to the satiation of phagocytic cells, to the saturation of the phagocytic mechanism, or to the depletion of opsonins or other serum factors by excessive amounts of colloidal material. The experiments reported indicate that processes are involved which are not readily accounted for by any of these mechanisms. Development of “blockade” was shown by Parker and Finney to occur after a 3- to 4-hr latent period. Four repeated intravenous injections of 6 mg of colloidal carbon given at 2-hr intervals to 25-gm mice prolong this latent period and frequently result in an increased rate of clearance. If the time interval is shortened to 1 hr, and, in addition, the dose of carbon is increased to 12 mg per injection, an even greater increase in colloid removal is observed. This very marked effect, the antithesis of blockade, is evidenced by a rate of removal many times as great as the rate of removal of the first injection. This phenomenon obviously occurs too rapidly to be the result of cellular proliferation, Indeed, it seems too rapid to be the result of synthesis of new opsonins and serum factors. The possibility was envisaged that phagocytic stimulation by particulate injection might provoke the elaboration of new protein for cell membrane or adaptive enzyme synthesis. However, neither actinomycin D nor puromycin treatment had any effect on the stimulation resulting from repeated injections of colloid.

Keywords

Latent Period Phagocytic Cell Repeated Injection Serum Factor Previous Injection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    B. Benacerraf, G. Biozzi, B.N. Halpern, and C. Stiffel, “Physiology of phagocytosis of particles by the RES.” In: B.N. Halpern, B. Benacerraf, and J. F. Delafresnaye, Eds., Physiopathology of the Reticuloendothelial System, A Symposium, Oxford, Blackwell, 1957, pp. 52–79.Google Scholar
  2. 2.
    B. Benacerraf, “Functions of the Kupffer cells.” In: Ch. Rouiller, Ed., The Liver II, New York, Academic Press, 1964, pp. 37–62.Google Scholar
  3. 3.
    E.L. Dobson, L.S. Kelly, C.R. Finney, and H.G. Parker, “Further complications in interpretation of RE system blockade.” Federation Proc., 22: 399, 1963 (Abstract).Google Scholar
  4. 4.
    C. R. Jenkin and D. Rowley, “The role of opsonins in the clearance of living and inert particles by cells of the reticuloendothelial system.” J. Exptl. Med., 114: 363–374, 1961.CrossRefGoogle Scholar
  5. 5.
    L. S. Kelly, E. L. Dobson, C. R. Finney, and J.D. Hirsch, “Proliferation of the reticuloendothelial system in the liver.” Am. J. Physiol., 198: 1134–1138, 1960.PubMedGoogle Scholar
  6. 6.
    I. M. Murray, “The effect of a plasma globulin fraction on the rate of phagocytosis.” Anat. Record, 139: 258–259, 1961 (Abstract).Google Scholar
  7. 7.
    I. M. Murray, “The mechanism of blockade of the reticuloendothelial system.” J. Exptl. Med., 117: 139–147, 1963.CrossRefGoogle Scholar
  8. 8.
    I. M. Murray, “Clearance rate in relation to agglutinins for gelatin-stabilized colloid in the rat.” Am. J. Physiol., 204: 655–659, 1963.PubMedGoogle Scholar
  9. 9.
    S.J. Normann and E. P. Benditt, “Reticuloendothelial blockade: The importance of the sequence and dose of administered particles. ” J. Reticuloendothelial Soc., 2: 345–346, 1965 (Abstract).Google Scholar
  10. 10.
    S.J. Normann and E.P. Benditt, “Function of the reticuloendothelial system, II. Participation of a serum factor in carbon clearance.” J. Exptl. Med., 122: 709–719, 1965.CrossRefGoogle Scholar
  11. 11.
    H. G. Parker and C. R. Finney, “Latent period in the induction of reticuloendothelial blockade.” Am. J. Physiol., 198: 916–920, 1960.PubMedGoogle Scholar
  12. 12.
    M.I. Arredondo and R. F. Kampschmidt, “Effect of endotoxins on phagocytic activity of the reticuloendothelial system of the rat.” Proc. Soc. Expt1. Biol. Med., 112: 78–81, 1963.Google Scholar

Copyright information

© Springer Science+Business Media New York 1967

Authors and Affiliations

  • Ernest L. Dobson
    • 1
  • Lola S. Kelly
    • 1
  • Caroline R. Finney
    • 1
  1. 1.Donner LaboratoryUniversity of CaliforniaBerkeleyUSA

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