Kinetics of the Phagocytosis of Repeated Injections of Colloidal Carbon: Blockade, a Latent Period or Stimulation? A Question of Timing and Dose
The phenomenon of “blockade” has been attributed to the satiation of phagocytic cells, to the saturation of the phagocytic mechanism, or to the depletion of opsonins or other serum factors by excessive amounts of colloidal material. The experiments reported indicate that processes are involved which are not readily accounted for by any of these mechanisms. Development of “blockade” was shown by Parker and Finney to occur after a 3- to 4-hr latent period. Four repeated intravenous injections of 6 mg of colloidal carbon given at 2-hr intervals to 25-gm mice prolong this latent period and frequently result in an increased rate of clearance. If the time interval is shortened to 1 hr, and, in addition, the dose of carbon is increased to 12 mg per injection, an even greater increase in colloid removal is observed. This very marked effect, the antithesis of blockade, is evidenced by a rate of removal many times as great as the rate of removal of the first injection. This phenomenon obviously occurs too rapidly to be the result of cellular proliferation, Indeed, it seems too rapid to be the result of synthesis of new opsonins and serum factors. The possibility was envisaged that phagocytic stimulation by particulate injection might provoke the elaboration of new protein for cell membrane or adaptive enzyme synthesis. However, neither actinomycin D nor puromycin treatment had any effect on the stimulation resulting from repeated injections of colloid.
KeywordsLatent Period Phagocytic Cell Repeated Injection Serum Factor Previous Injection
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