31P NMR Spectroscopy of Brain and Heart
It was in 1974 when Hoult first applied NMR techinque to measure metabolites containing phosphorous in rat muscle. Moon and Richards demonstrated that 31P NMR can be used to measure intracellular Ph. They were great steps forward in understanding the fine regulation of cellular energy metabolism in comparison to the classical biochemical techniques, since the method is nondestructive and it gives a continuous monitoring of phosphate metabolites. Using this method, the concentrations of ATP, phosphocreatine, sugar phosphate, and inorganic phosphates were monitored on frog gastrocnemius (Burt, Dawson, Gadian), isolated hearts and recently on all the perfusable organs under different conditions (Gadian, Nunnally, Cohen). The first in vivo 31P NMR experiment was carried out by Chance et al, who demonstrated the possibility of monitoring the levels of phosphorous containing metabolites of brain tissue in vivo. All these studies were done with relatively small bore magnets (1–3cm) limiting the extent of these experiments.
KeywordsSugar Rubber Neurol Ketamine Phosphocreatine
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