Oxygen Indicator Dilution Curves of the Canine Cerebral Circulation

  • P. Grieb
  • R. E. Forster
  • P. C. Pape
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 159)


Indicator-dilution methodology is a useful tool in studies of transcapillary exchange (6,10), but oxygen isotopes have seldom been used in such studies, because they require non-standard laboratory techniques. Of the two isotopes of oxygen currently used in physiological research, positron-emitting oxygen-15 is not suitable for the indicator-dilution technique, because one cannot distinguish between signal originating from unmetabolized 15O2, and that from metabolically-formed H2 15O molecules (28). The other is the stable isotope oxygen-18, which can be detected by mass spectrometric techniques. Tb our knowledge, there are only two preliminary reports of stable oxygen tracer dilution curves. Chinard et al, (8) published a 18O2 dilution curve of kidney, with 51Crlabelled red blood cells (Cr-rbc) as the reference indicator. In that experiment, the labelled oxygen curve preceeded the reference curve, which was attributed to the existence of a diffusional shunt in kidney. Forster et al, (13) obtained 18O2 dilution curves of skinned canine hind limb, with indocyanine green as the reference tracer. In these experiments, labelled oxygen curves lagged behind the reference.


Cerebral Blood Volume Reference Curve Dilution Curve Oxygen Distribution Reference Indicator 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • P. Grieb
    • 1
  • R. E. Forster
    • 1
  • P. C. Pape
    • 1
  1. 1.Department of PhysiologyUniversity of PennsylvaniaPhiladelphiaUSA

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