Calcium-Induced Modulation of the Tubulin Pool in Parathyroid Glands

  • C. A. Bader
  • J. D. Monet
  • J. L. Funck-Brentano
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 103)


Participation of cytoplasmic microtubules has been implicated in various secretory processes such as release of hormone from secretory cells and secretion or movement of granular products (see ref. 1, 2 for review). The great bulk of these works is based on the inhibitory effects of colchicine (3) and vinca-alkaloids (4). The mechanism of action has been attributed to the ability of these agents to bind to tubulin, the protein subunit of the cellular microtubules, and as a consequence, to prevent microtubule assembly. With these techniques it has been shown that colchicine and vinca-alkaloids may affect Parathyroid Hormone (PTH) secretion in in vitro culture of Parathyroid Glands (PTG) (5, 6). It has been suggested that these effects may indicate a role of microtubules in the sequence of events leading to release of PTH. Nevertheless although ultrastructural studies of PTG have demonstrated the presence of microtubules (6, 7, 8, 9), anatomical evidence to support a mechanism by which microtubules might affect intracellular processing of PTH are rare. On the other hand in in vivo studies the precise site of action of colchicine is difficult to ascertain because it has been shown that this product may produce hypocalcemia (10) and may inhibit bone resorption (11). Moreover recent studies have shown that the renal handling of phosphate and its regulation by PTH may depend on cytoplasmic microtubules, which have been described in proximal renal tubule cells (12). To further assess the presence of a microtubular system (MTS) in PTG and its possible functional significance, we initiated the following studies which extend previous work in our laboratory (13) in order to demonstrate tubulin in PTG on a biochemical basis. After demonstration of a specific Colchicine Binding Protein (Co1BP) in PTG, the tubulin pool size level in PTG slices incubated in vitro in various extracellular calcium concentrations was assayed.


Parathyroid Hormone Parathyroid Gland Extracellular Calcium Concentration Precise Site Cytoplasmic Microtubule 
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  1. 1.
    The Biology of Cytoplasmic Microtubules. D. Soifer (Ed.), Annals of the New York Academy of Sciences, Volume 253, 1975.Google Scholar
  2. 2.
    Wolff, J. and Williams, J.A.: The Role of Microtubules and Microfilaments in Thyroid Secretion. Rec. Prog. Horm. Res. 29: 229, 1973.PubMedGoogle Scholar
  3. 3.
    Borisy, G.G. and Taylor, E.W.: The Mechanism of Action of Colchicine. J. Cell. Biol. 34: 525, 1967.PubMedCrossRefGoogle Scholar
  4. 4.
    Wilson, L.: Properties of Colchicine Binding Protein from Chick Embryo Brain. Interactions with Vinca-Alkaloids and Podophyllotoxin. Biochemistry: 9, 4999, 1970.PubMedCrossRefGoogle Scholar
  5. 5.
    Chertow, B.S, Williams, G.A., Kiani, R, Stewart, K.L., Hargis, G.K. and Flayter, R.L.: The Interactions Between Vitamin A, Vinblastine, and Cytochalasin B in Parathyroid Hormone Secretion. Proc. Soc. Exp. Biol. Med. 147: 16, 1974.PubMedGoogle Scholar
  6. 6.
    Chertow, B.S., Manke, D.J., Williams, G.A., Baker, G.R., Hargis, G.K. and Buschmann, R.J.: Secretory and Ultrastructural Responses of Hyperfunctioning Human Parathyroid Tissues to Varying Calcium Concentration and Vinblastine. Lab. Invest. 36: 198, 1967.Google Scholar
  7. 7.
    Chertow, B.S., Buschmann, R.J. and Henderson, W.J.: Subcellular Mechanisms of Parathyroid Hormone Secretion. Lab. Invest. 32: 190, 1975.PubMedGoogle Scholar
  8. 8.
    Reaven, E.P. and Reaven G.M.: A Quantitative Ultrastructural Study of Microtubule Content and Secretory Granule Accumulation in Parathyroid Glands of Phosphate-and Colchicine- Treated Rats. J. Clin. Invest. 56: 49, 1975.PubMedCrossRefGoogle Scholar
  9. 9.
    Reaven, G.M, Reaven, P.D. and Reaven, E.P.: Hypercalcemia in Acute Uremia and Following Citric Acid Administration: Differential Effect on Parathyroid Gland Microtubule Cdntent. Metabolism, 25: 203, 1976.PubMedCrossRefGoogle Scholar
  10. 10.
    Heath, D.A., Palmer, J.S. and Aurbach, G.D.: The Hypocalcemic Action of Colchicine. Endocrinology, 90: 1589, 1972.PubMedCrossRefGoogle Scholar
  11. 11.
    Raisz, L.G., Holtrop, M.E. and Simmons, H.A.: Inhibition of Bone Resorption by Colchicine in Organ Culture. Endocrinology, 92: 556, 1973.PubMedCrossRefGoogle Scholar
  12. 12.
    Dousa, T.P., Duarte, C.G. and Knox, F.G.: Effect of Colchicine on Urinary Phosphate and Regulation by Parathyroid Hormone. Amer. J. Physiol. 231: 61, 1976.PubMedGoogle Scholar
  13. 13.
    Bader, C., Monet, J.D., Chanard, J. and Funck-Brentano, J.L.: Mise en évidence d’une protéine microtubulaire (Tubuline) dans le cytosol de la parathyroïde de porc. C.R. Acad. Sc. Paris, 282: 2099, 1976.Google Scholar
  14. 14.
    Zweig, M.H. and Chignell, C.F.: Interaction of some Colchicine Analogs, Vinblastine and Podophyllotoxin with Rat Brain Micro-tubule Protein. Biochem. Pharmacol. 22: 2141, 1973.PubMedCrossRefGoogle Scholar
  15. 15.
    Eipper, B.A.: Rat Brain Microtubule Protein:Purification and Determination of Covalently Bound Phosphate and Carbohydrate. Proc. Nat. Acad. Sci. U.S.A. 69: 2283, 1972.CrossRefGoogle Scholar
  16. 16.
    Pipeleers, D.G., Pipeleers-Marichal, M.A. and Kipnis, D.M.: Microtubule Assembly and the Intracellular Transport of Secretory Granules in Pancreatic Islets. Science, 191: 88, 1976.PubMedCrossRefGoogle Scholar
  17. 17.
    Kemper B., Habener J.F., Rich, A and Potts, J.T., Jr.: Micro-tubules and the Intracellular Conversion of Proparathyroid Hormone to Parathyroid Hormone. Endocrinology, 96: 903, 1975.PubMedCrossRefGoogle Scholar
  18. 18.
    Maetz, J. and Pic, P. Microtubules in the “Chloride Cell” of the Gill and Disrupting Effects of Colchicine on the Salt-Balance of the Sea-Water Adapted Mugil Capito. J. Exp. Zool. 199: 325, 1976.CrossRefGoogle Scholar
  19. 19.
    Solomon, F.: Binding Sites for Calcium on Tubulin. Biochemistry, 16: 358, 1977.PubMedCrossRefGoogle Scholar
  20. 20.
    Schliwa, M.: The Role of Divalent Cations in the Regulation of Microtubule Assembly. J. Cell. Biol. 70: 527, 1976.PubMedCrossRefGoogle Scholar
  21. 21.
    Olmsted, J.B. and Borisy, G.G.: Ionic Nucleotide Requirements for Microtubule Polymerization in Vitro. Biochemistry, 14: 2996, 1975.PubMedCrossRefGoogle Scholar
  22. 22.
    Matsuzaki, S. and Dumont, J.E.: Effect of Calcium Ion on Horse Parathyroid Gland Adenyl Cyclase. Biochim. Biophys. Acta. 284: 227, 1972.PubMedCrossRefGoogle Scholar
  23. 23.
    Williams, G.A., Hargis, G.K., Bowser, E.N., Henderson, W.J. and Martinez, N.J.: Evidence for a Role of Adenosine 3’, 5’-Monophosphate in Parathyroid Hormone Release. Endocrinology, 92: 687, 1973.PubMedCrossRefGoogle Scholar
  24. 24.
    Gillespsie, E.: Microtubules, Cyclic AMP, Calcium, and Secretion. In Ref. 1, p. 771.Google Scholar
  25. 25.
    Raisz, L.G.: Effects of Calcium on Uptake and Incorporation of Amino Acids in the Parathyroid Glands. Biochim. Biophys. Acta. 148: 460, 1967.PubMedCrossRefGoogle Scholar
  26. 26.
    Kemper, B., Habener, J.F., Rich, A. and Potts, J.T., Jr.: Parathyroid Secretion: Discovery of a Major Calcium-Dependent Protein. Science, 184: p. 167, 1974.PubMedCrossRefGoogle Scholar
  27. 27.
    Weisenberg, R.C., Borisy, G.G. and Taylor, E.W.: The Colchicine-Binding Protein of Mammalian Brain and its Relation to Microtubules. Biochemistry, 7: 4466, 1968.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • C. A. Bader
    • 1
  • J. D. Monet
    • 1
  • J. L. Funck-Brentano
    • 1
  1. 1.INSERM U.90Hôpital NeckerParis Cedex 15France

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