Bone Lining Cells and the Bone Fluid Compartment, an Ultrastructural Study

  • J. L. Matthews
  • C. Vander Wiel
  • R. V. Talmage
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 103)


Critical data has been developed in recent years that supports the hypothesis that ion concentrations in bone fluid and general extracellular fluid are modulated by a functional membrane that separates the fluid of bone from the general ECF. The evidence for the postulated functional membrane in bone is based on the experiments of Geisler and Neuman1, and Ramp and Neuman2, Scarpace and Neuman3,4. The prime points of the data are that potassium is concentrated in the bone fluid and that mineralization of the matrix is increased when bone cells are disrupted or poisoned inferring that the cells serve to partition these fluids, excluding excess calcium from the matrix surfaces. The actual “membrane” and mechanisms of its action are not clearly established. Talmage has suggested that the cells lining the bone surface (osteoblasts) serve this partitioning function5. The Talmage model proposes that calcium enters the bone fluid compartment by passive diffusion from the general ECF to the bone fluid compartment by passing between the lining cells. In the Talmage model, calcium eflux from the bone occurs by entering the osteocytes and osteoblasts whereupon it is actively transported through the cells and through the plasma membrane to reach the general ECF. Entry into the cell would not be difficult as a downhill gradient would exist, i.e., intracellular calcium levels are approximately 10–5.


Bone Cell Endosteal Bone Functional Membrane Bone Membrane Bone Lining Cell 
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  1. 1.
    Geisler, J.A., and Neuman, W.F.: The Membrane Control of Bone Potassium. Por. Soc. Exptl. Biol. Med. 130: 608, 1969.Google Scholar
  2. 2.
    Ramp, W.K., and Neuman, W.F.: Some Factors Affecting Mineralization of Bone In Tissue Culture. Am. J. Physiol. 220: 270, 1971.PubMedGoogle Scholar
  3. 3.
    Scarpace, P.J. and Neuman, W.F.: Quantitation of Ca Fluxes in Chick Calvaria. Biochem. Biophys. Acta (Amst).Google Scholar
  4. 4.
    Scarpace, P.J. and Neuman, W.F.: The Blood: Bone Disequilibrium. Calcif. Tiss. Res. 20: 151, 1976.CrossRefGoogle Scholar
  5. 5.
    Talmage, R.V.: Calcium Homeostasis-Calcium Transport-Parathyroid Action. Clin. Orthop. 67: 210, 1969.Google Scholar
  6. 6.
    Neuman, W.F., Brommage, R.J., and Neuman, M.W.: Aerobic Glyeolysis, Ion Fluxes, and Bone Membranes, Proceedings of 6th Parathyroid Conference, 1977. in press.Google Scholar
  7. 7.
    Matthews, J.L., Martin, J.H., Collins, E.J., Kennedy, J.W., and Powel, E.L., Jr.: Immediate Changes in the Ultra-structure of Bone Cells Following Thyrocalcitonin Administration. In: Calcium, Parathyroid Hormone and the Calcitonins. Excerpta Medica. p. 375, 1972.Google Scholar
  8. 8.
    Davis, W.L., Matthews, J.L., Martin, J.H., Kennedy, J.W., and Talmage, R.V.: The Endostream as a Functional Membrane. In: Calcium Regulating Hormones, Excerpta Medica, p. 275, 1975.Google Scholar
  9. 9.
    Boyde, A.: S.E.M. Studies on Bone Cells in Culture, Proceedings of Sixth Parathyroid Conference. Excerpta Medica, in press.Google Scholar
  10. 10.
    Miller, G., Davis, W.L., Jones, R.G., Jones, J.L., and Matthews, J.L.: Intact Endosteal Membranes to Study Bone Cell Physiology: A Scanning Electron Microscopic and Metabolic Study of the Rat Endosteum. Anat. Rec., in press.Google Scholar
  11. 11.
    Jones, S.J., and Boyde, A.: Morphological Changes of Osteoblasts in vitro. Cell Tiss. Res. 166: 101, 1976.CrossRefGoogle Scholar
  12. 12.
    Jones, J.L., Davis, W.L., Miller, G.W., Jones, R.G., and Matthews, J.L.: The Effect of Cytochalasin B on the Endosteal Lining Cells of Mammalian Bone: A Scanning Electron Microscopic Study. Cell. Biol. in press.Google Scholar
  13. 13.
    Brighton, C.T., and Hunt, R.M.: Histochemical Localization of Calcium in Growth Plate Mitochondria and Matrix Vesicles. Fed. Proc. 35: 143, 1976.PubMedGoogle Scholar
  14. 14.
    Vander Wiel, C., Matthews, J.L., and Talmage, R.V.: Intracellular Calcium Localization in Cells Lining Bone Surfaces Following Parathyroid Hormone Injection. Proceedings of Sixth Parathyroid Conference. Excerpta Medica. in press.Google Scholar
  15. 15.
    Matthews, J.L., Davis, W.L., Martin, J.H., and Talmage, R.V.: The Endosteal Cell Response to Exogenous Stimuli, An Electron Microscope Study. In: Extracellular Matrix Influences on Gene Expression. Academic Press. p. 735, 1975.Google Scholar
  16. 16.
    Talmage, R.V., Matthews, J.L., Martin, J.H., Kennedy, J.W., Davis, W.L., and Roycroft, J.H.: Calcium, Phosphate and the Osteocyte-Osteoblast Bone Cell Unit. In: Calcium Regulating Hormones. Excerpta Medica, p. 284, 1974.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • J. L. Matthews
    • 1
    • 2
  • C. Vander Wiel
    • 1
    • 2
  • R. V. Talmage
    • 1
    • 2
  1. 1.Department of Pathology, Department of Orthopedic SurgeryBaylor University Medical CenterDallasUSA
  2. 2.University of North CarolinaChapel HillUSA

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