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Low Phosphorus Intake and Vitamin D Metabolism and Expression in Rats

  • S. Edelstein
  • D. Noff
  • J. Puschett
  • E. E. Golub
  • F. Bronner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 103)

Abstract

Male weanling rats, depleted of vitamin D for 4 wks., were repleted for 4 wks. with 1,2 — [3H]-4-[14C]-vitamin D3 and then placed on three regimens: normal mineral intake (0.4% Ca, 0.3% P); low-Ca (0.004% Ca, 0.3% P) and low-P (0.4% Ca, 0.13% P). Three weeks later 1,25-dihydroxyvitamin D3 (1,25-(OH)2-D3) levels were determined in plasma and intestine on the basis of the 3H/14C radioactivity ratios. Calcium-binding protein (CaBP) was estimated in parallel experiments with 4 groups of rats that had been on semi-synthetic regimens containing 1.5% Ca, 1.5% P; 1.5% Ca, 0.2% P; 0.06% Ca, 0.2% P; and 1.5% Ca, 0.03% P for 7–10 days. In comparison with the controls, the low Ca diets Zed to a doubling in 1,25-(OH)2-D3 levels in intestinal mucosa, and to detectable levels of 1,25-(OH)2-D3 in plasma. CaBP was markedly elevated. On the other hand, in the low P groups, in spite of hypophosphatemia, 1,25-(OH)2-D3 was undetectable in plasma and virtually undetectable in intestine. Moreover, CaBP levels were unchanged on 0.2% P intakes and markedly depressed on 0.03% P. Hypophosphatemia became more pronounced as P intake decreased, while plasma Ca increased. It therefore appears that in rats, as contrasted with chickens, hypophosphatemis is not a major factor regulating metabolism and expression of 1,25-(OH)2-D3.

Keywords

Intestinal Mucosa Increase Calcium Absorption Radioactivity Ratio Intestinal Calcium Transport Rachitic Chick 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • S. Edelstein
    • 1
  • D. Noff
    • 1
  • J. Puschett
    • 2
  • E. E. Golub
    • 3
  • F. Bronner
    • 3
  1. 1.Municipal Government Medical CenterIchilov HospitalTel Aviv -JaffoIsrael
  2. 2.Allegheny General HospitalPittsburghUSA
  3. 3.The University of Connecticut Health CenterFarmingtonUSA

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