Low Phosphorus Intake and Vitamin D Metabolism and Expression in Rats

  • S. Edelstein
  • D. Noff
  • J. Puschett
  • E. E. Golub
  • F. Bronner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 103)


Male weanling rats, depleted of vitamin D for 4 wks., were repleted for 4 wks. with 1,2 — [3H]-4-[14C]-vitamin D3 and then placed on three regimens: normal mineral intake (0.4% Ca, 0.3% P); low-Ca (0.004% Ca, 0.3% P) and low-P (0.4% Ca, 0.13% P). Three weeks later 1,25-dihydroxyvitamin D3 (1,25-(OH)2-D3) levels were determined in plasma and intestine on the basis of the 3H/14C radioactivity ratios. Calcium-binding protein (CaBP) was estimated in parallel experiments with 4 groups of rats that had been on semi-synthetic regimens containing 1.5% Ca, 1.5% P; 1.5% Ca, 0.2% P; 0.06% Ca, 0.2% P; and 1.5% Ca, 0.03% P for 7–10 days. In comparison with the controls, the low Ca diets Zed to a doubling in 1,25-(OH)2-D3 levels in intestinal mucosa, and to detectable levels of 1,25-(OH)2-D3 in plasma. CaBP was markedly elevated. On the other hand, in the low P groups, in spite of hypophosphatemia, 1,25-(OH)2-D3 was undetectable in plasma and virtually undetectable in intestine. Moreover, CaBP levels were unchanged on 0.2% P intakes and markedly depressed on 0.03% P. Hypophosphatemia became more pronounced as P intake decreased, while plasma Ca increased. It therefore appears that in rats, as contrasted with chickens, hypophosphatemis is not a major factor regulating metabolism and expression of 1,25-(OH)2-D3.


Intestinal Mucosa Increase Calcium Absorption Radioactivity Ratio Intestinal Calcium Transport Rachitic Chick 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • S. Edelstein
    • 1
  • D. Noff
    • 1
  • J. Puschett
    • 2
  • E. E. Golub
    • 3
  • F. Bronner
    • 3
  1. 1.Municipal Government Medical CenterIchilov HospitalTel Aviv -JaffoIsrael
  2. 2.Allegheny General HospitalPittsburghUSA
  3. 3.The University of Connecticut Health CenterFarmingtonUSA

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