Importance of 25-Hydroxylation to the Renal Tubular Actions of Vitamin D Metabolites

  • Jules B. Puschett
  • John Szramowski
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 103)


Experimental observations from my own laboratory as well as data from other workers have documented an acute effect of vitamin D and certain of its metabolites on renal tubular electrolyte transport (1–5). Both in the dog and the rat, the infusion of the biologically active derivative of vitamin D3, 25-hydroxycholecalciferol (25 HCC) and 1,25-dihydroxycholecalciferol (1,25 DHCC) has been demonstrated to produce an acute enhancement of phosphate (1–5), calcium and sodium (4,5) reabsorption. Recently, a series of additional metabolites and analogues has been discovered (6). Their subsequent characterization and synthesis have provided us with the opportunity to study the structural requirements of these vitamin D3 derivatives with regard to their ability to alter renal tubular transport. The protocol utilized for these studies was identical to that originally described (1).


Effective Renal Plasma Flow Phosphate Excretion Renal Tubular Transport Allegheny General Hospital Vasopressin Administration 
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  1. 1.
    Puschett, J.B., Moranz, J. and Kurnick, W.S.: Evidence for a direct action of cholecalciferol and 25-hydroxycholecalciferol on the renal transport of phosphate, sodium and calcium. J. Clin. Invest. 51: 373, 1972.PubMedCrossRefGoogle Scholar
  2. 2.
    Puschett, J.B., Fernandez, P.C., Boyle, I.T., Gray, R.W., Omdahl, J.L. and DeLuca, H.F.: The acute renal tubular effects of 1,25-dihydroxycholecalciferol. Proc. Soc. Exp. Biol. Med. 141: 379, 1972.PubMedGoogle Scholar
  3. 3.
    Puschett, J.B., Beck, W.S.,Jr., Jelonek, A. and Fernandez, P.C.: Study of the renal tubular interactions of thyrocalcitonin, cyclic adenosine 3’,5’-monophosphate, 25-hydroxycholecalciferol, and calcium ion. J. Clin. Invest. 53: 756, 1974.PubMedCrossRefGoogle Scholar
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    DeLuca, H.F.: Vitamin D endocrinology. Ann. Int. Med. 85: 367, 1976.PubMedGoogle Scholar
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    DeLuca, H.F.: Metabolism of vitamin D: current status. Am. J. Clin. Nutr. 29: 1258, 1976.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Jules B. Puschett
    • 1
    • 2
  • John Szramowski
    • 1
    • 2
  1. 1.Allegheny General HospitalUniversity of Pittsburgh Medical SchoolPittsburghUSA
  2. 2.PittsburghUSA

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