High-Performance Liquid Chromatographic Analysis of Glycosphingolipids and Phospholipids
The modern form of liquid chromatography commonly referred to as high-speed or high-performance liquid chromatography (HPLC) has developed very rapidly in the past few years. HPLC implies the use of reusable columns, injection port sample application, the use of pumps for uniform solvent flow at high-pressure if necessary and automatic sample detection. Since 1696–1970 a better understanding of the factors which improve the speed and resolution in liquid chromatography has evolved. New column packing materials, column packing techniques, high pressure — low volume equipment and highly sensitive detectors have been developed. A large variety of such columns and equipment are now available commercially. The modern HPLC techniques have led to the development of sensitive, quantitative methods, analogous to that available for volatile materials by gas-chromatography, for a very large variety of relatively high molecular weight substances of biological interest.
KeywordsBenzoyl Chloride Performance Liquid Chromatographic Analysis Sphingomyelinase Activity High Molecular Weight Substance Benzoic Anhydride
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