High-Performance Liquid Chromatographic Analysis of Glycosphingolipids and Phospholipids

  • Robert H. McCluer
  • Firoze B. Jungalwala
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 68)


The modern form of liquid chromatography commonly referred to as high-speed or high-performance liquid chromatography (HPLC) has developed very rapidly in the past few years. HPLC implies the use of reusable columns, injection port sample application, the use of pumps for uniform solvent flow at high-pressure if necessary and automatic sample detection. Since 1696–1970 a better understanding of the factors which improve the speed and resolution in liquid chromatography has evolved. New column packing materials, column packing techniques, high pressure — low volume equipment and highly sensitive detectors have been developed. A large variety of such columns and equipment are now available commercially. The modern HPLC techniques have led to the development of sensitive, quantitative methods, analogous to that available for volatile materials by gas-chromatography, for a very large variety of relatively high molecular weight substances of biological interest.


Benzoyl Chloride Performance Liquid Chromatographic Analysis Sphingomyelinase Activity High Molecular Weight Substance Benzoic Anhydride 
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  1. 1.
    McCluer, R.H. and Evans, J.E.: Preparation and analysis of benzoylated cerebrosides. J. Lipid Res., 14: 611, 1973.PubMedGoogle Scholar
  2. 2.
    Sugita, M., Iwamori, M., Evans, J., McCluer, R.H., Moser, H.W. and Dulaney, J.T.: High-performance liquid chromatography of ceramides: application to analysis in human tissues and demonstration of ceramide excess in Faber’s disease. J. Lipid Res., 15: 223, 1974.PubMedGoogle Scholar
  3. 3.
    McCluer, R.H. and Evans, J.E.: Quantitative analysis of cerebroside by high-performance liquid chromatography of their perbenzoyl derivatives. J. Lipid Res., submitted.Google Scholar
  4. 4.
    Hayes, L., Jungalwala, F.B. and McCluer, R.H.: Determination of picomole quantities of cerebrosides by high-performance liquid chromatography with gradient elution analysis. In preparation.Google Scholar
  5. 4a.
    Hoshi, M., Williams, M. and Kishimoto, K.: Characterization of brain cerebrosides at early stages of development in the rat. J. Neurochem., 21: 709, 1973.PubMedCrossRefGoogle Scholar
  6. 5.
    Kishimoto, Y. and Mitry, M.I.T.: A new procedure for synthesis of 3-keto derivatives of sphingolipids and its application for study of fatty acid composition of brain ceramides and cerebrosides containing dihydro-sphingosine or sphingosine. Arch. Biochem. Biophys., 161: 426, 1974.PubMedCrossRefGoogle Scholar
  7. 6.
    Iwamori, M., Moser, H.W., McCluer, R.H. and Kishimoto, Y.: 2-ketosphingolipids: Application to the determination of sphingolipids which contain 4-sphingenine. Biochem. Biophys. Acta, 380: 308, 1975.PubMedGoogle Scholar
  8. 7.
    Jungalwala, F.B., Turel, K.J., Evans, J.E. and McCluer, R.H.: Sensitive analysis of ethanolamine and serine containing phosphoglycerides by high performance liquid chromatography. Biochem. J., 145: 517, 1975.PubMedGoogle Scholar
  9. 8.
    Dittmer, J.C. and Wells, M.A.: Quantitative and qualitative analysis of lipids and lipid components. Methods in Enzym., 14: 482, 1969.CrossRefGoogle Scholar
  10. 9.
    Arvidson, G.A.E.: Structural and metabolic heterogeneity of rat liver glycerophosphatides. European J. Biochem., 4: 478, 1968.CrossRefGoogle Scholar
  11. 10.
    Avidson, G.A.E.: Separation of naturally occurring lecithins according to fatty-acid chain-length and degree of unsaturation on a lipophilic derivative of Sephadex. J. of Chromatography, 103: 201, 1975.CrossRefGoogle Scholar
  12. 11.
    Wurster, Jr., C.F. and Copenhaver, Jr., J.H.: Thin layer chromatographic separation of dimethylphosphatidates derived from lecithins. Lipids, 1: 422, 1966.PubMedCrossRefGoogle Scholar
  13. 12.
    Renkonen, O.: Mono-acid dimethyl phosphatidates from different subtypes of choline and ethanolamine glycerophosphatides. Biochim. Biophys. Acta, 152: 114, 1968.PubMedGoogle Scholar
  14. 13.
    Kampine, J.P., Brady, R.O., and Kanfer, J.N.: Diagnosis of Gaucher’s disease and Niemarui-Pick disease with small samples of nervous blood. Science, N.Y., 155: 86, 1967.CrossRefGoogle Scholar
  15. 14.
    Gal, A.E., Brady, R.O., Hibbert, S.R. and Pentchev, P.G.: A practical chromatogenic procedure for the detection of homozygotes and heterozygous carriers of Niemann-Pick disease. New Engl. J. Med., 293, 632, 1975.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Robert H. McCluer
    • 1
  • Firoze B. Jungalwala
    • 1
  1. 1.Department of BiochemistryEunice Kennedy Shriver Center at Walter E. Fernald State SchoolWalthamUSA

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