Sensitivity and Specificity of Prostanoic Derivatives Radioimmunoassays: New Strategy

  • Fernand Dray
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 44)


Prostanoic derivatives, as prostaglandin, thromboxane, prostacyclin and their metabolites, are generally present in biological media at very low concentrations (10−9–10−11 M). Therefore assays with high sensitivity often are required for their measurement. Two techniques are widely used: mass spectrometry combined with gas chromatography (GS-MS), which is particularly specifics1 and radio-immunoassay (RIA) which is particularly sensitive and convenient for large series2,7. In our laboratory, immunological methods have been developed using radioactive8 or non-radioactive tracers5,9; iodinated derivatives (as PG−125I-Histamine) have been shown to be more sensitive convenient 10–14, In this paper some aspects of the use of iodinated tracers will be presented and a new strategy for increasing the specificity of RIA of prostanoic derivatives will be proposed.


High Performance Liquid Chromatography Silicic Acid Silicic Acid Column Meclofenamic Acid Silicic Acid Chromatography 
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Copyright information

© Springer Science+Business Media New York 1982

Authors and Affiliations

  • Fernand Dray
    • 1
  1. 1.Fra n° 8 InsermURIA Institut PasteurParis Cedex 15France

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