Sensitivity and Specificity of Prostanoic Derivatives Radioimmunoassays: New Strategy
Prostanoic derivatives, as prostaglandin, thromboxane, prostacyclin and their metabolites, are generally present in biological media at very low concentrations (10−9–10−11 M). Therefore assays with high sensitivity often are required for their measurement. Two techniques are widely used: mass spectrometry combined with gas chromatography (GS-MS), which is particularly specifics1 and radio-immunoassay (RIA) which is particularly sensitive and convenient for large series2,7. In our laboratory, immunological methods have been developed using radioactive8 or non-radioactive tracers5,9; iodinated derivatives (as PG−125I-Histamine) have been shown to be more sensitive convenient 10–14, In this paper some aspects of the use of iodinated tracers will be presented and a new strategy for increasing the specificity of RIA of prostanoic derivatives will be proposed.
KeywordsHigh Performance Liquid Chromatography Silicic Acid Silicic Acid Column Meclofenamic Acid Silicic Acid Chromatography
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- 1.K. Grenn, M. Hamberg, and B. Samuelsson. Quantitative analysis of prostaglanin and thromboxanes by mass spectrometric methods. In “Advances in Prostaglandin and Thromboxane Research”, B. Samuelsson and R. Paoletti Ed., Raven Press. N.Y. p. 47 (1976).Google Scholar
- 4.B.M. Jaffe, J.W. Smith, W.Y. Newton, and C.W. Parker. Radio-immunoassay for prostaglandins. Science 171:494 (1971) 162 F. DRAYGoogle Scholar
- 7.F. Dray, and B. Charbonnel. Dosage radioimmunologique des prostaglandines Fa et El dans le plasma périphérique de l’homme normal. Colloque INSERM, p. 133 (1973)Google Scholar
- 14.F. Dray, K. Gerozissis, B. Kouznetzova, S. Mama, P. Pradelles, and G. Trugnan. New approach to the RIA of prostaglandins and related compounds using iodinated tracers. In: “Advances in Prostaglandin and Thromboxane Research”, B. Samuelsson, P.W. Ramwell and R. Paoletti Ed., Raven Press, N.Y. p. 167 (1980).Google Scholar
- 16.W. M. Hunter, and F.C. Greenwood. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature (London) 194: 495 (1962).Google Scholar