Studies of Regulation of Hexose Transport into Cultured Fibroblasts
The animal cells under discussion are cultured avian and mammalian fibroblasts in the postlogarithmic growth phase (or in tumorigenic cultures, medium to high density). The cultures are monolayer cultures, not suspension cultures.
The main theme will be the effects of carbohydrates and their metabolism in relation to the regulation of uptake or transport of hexoses into cultured fibroblasts, including special metabolic mutants. The downregulation of the hexose transport of the cultures will be called “the glucose-mediated transport curb.”
The transport systems discussed are largely the facilitated diffusion of hexoses and its regulation by carbohydrate metabolism. Where amino acid transport systems (such as the A and L systems) are discussed, this is strictly related to carbohydrate transport and metabolism.
Since hexose transporters are membrane proteins, aspects of protein recycling will have to be considered, and hence also the effects of inhibitors of protein synthesis.
Some stress-related factors, such as glucose starvation, uncouplers of respiration, as well as oncological transformations and their effects on hexose transport regulation, will be discussed.
KeywordsChicken Embryo Fibroblast Rous Sarcoma Virus Hexose Transport Glucose Starvation Amino Acid Transport System
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