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Usefulness of Commercially Available Media to MIC-Determinations of Trimethoprim

  • B. van Klingeren
  • A. Rutgers
Part of the Chemotherapy book series (CT, volume 2)

Abstract

Since trimethoprim and cotrimoxazole came into use several authors have pointed out the presence of antagonists to TMP in most commercially prepared media. In 1971 Koch and Burchall showed that thymidine was the most important TMP-antagonist in those media. Amyes and Smith (1974) recently compared several media, among others Oxoid Diagnostic Sensitivity Test Agar and Wellcotest Agar; they observed that DST supported the growth of thymineless mutants; the suggestion that this medium contained thymine and/or thymidine was confirmed by sensitivity-testing indicating that DST was unsatisfactory for IMP-sensitivity-testing; they also showed that Wellcotest Agar contains a uridinelike compound and that this prevents the utilisation of thymine (not of thymidine) by thymineless mutants.

Keywords

Minimal Inhibitory Concentration Open Circle Sensitivity Testing Complete Inhibition Dilution Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Amyes, S.G.B. and I.T.Smith. Trimethoprim-sensitivity testing and thymineless mutants. J.Med.Microbiol. 7 (1974) 143–153.Google Scholar
  2. Koch, A.E. and J.J.Burchall. Reversal of the antimicrobial activity of trimethoprim by thymidine in commercially prepared media. Appl.Microbiol. 22 (1971) 812–817.PubMedGoogle Scholar
  3. Then, R. Mechanismus der bakteriziden Wirkung von Folatantagonisten. Zbl.Dakt.Hyg., I.Abt.Orig.A 228 (1974) 273–277.Google Scholar

Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • B. van Klingeren
    • 1
  • A. Rutgers
    • 1
  1. 1.National Institute of Public HealthBilthovenThe Netherlands

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