Chronic E.coli Nephritis in Rats: Model for Assessment of Activity of Antimicrobial Agents
Antibacterial activity of chemotherapeutic drugs is preferably tested in acute animal infections. In contrast, the prevailing clinical problem is therapy of chronic local infections. For this reason an animal model has been developed which takes a chronic course for months by cautious impairment of systemic and local defence mechanisms, and thus allows delayed therapy of a well established infection. Results of experimental chemotherapy of hormonally induced chronic E.coli nephritis in rats are domonstrated to be significantly inferior to results with immediate treatment of acute renal infections. The method of this animal model and its applicability for the assessment of antimicrobial agents are presented.
Animal experiments, besides being used for exploration of properties of bacteria and of host defence mechanisms, were preferably applied for assessment of the effectiveness of chemotherapeutic drugs. For this purpose renal infection models have gained more and more interest and preference in the past years. As far as the mode of infection causes a massive impairment of the urinary tract (Prát et al. 1968) or even of the kidneys (Miller and Robinson 1973) difficulties arise if prolonged observation of the experiments is intended: this means, those infections tend to heal spontaneously or,- mostly due to superinfections — tend to kill the animals. Therefore it turned out to be neccessary in all these types of animal models to start with therapy very early in the course of infection, i.e. in the acute phase of infection.
The model of hormonally facilitated pyelonephritis developed in our group doesn’t give rise to disadvantages mentioned above. This procedure has proved to be of great value for investigations of pathogenetic relationships, and especially for therapeutic studies in infections already persistant.
KeywordsChemotherapeutic Drug Bacterial Count Interstitial Nephritis Established Infection Renal Infection
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