Pathogenesis of an Experimental Pyelonephritis Model in the Mouse and Its Use in the Evaluation of Antibiotics
Intravenous inoculation of Escherichia coli, Proteus morganii and Pseudomonas aeruginosa into healthy mice failed to produce infections of the kidneys. Large inocula of cells caused death of the animals without obvious involvement of the kidneys while small inocula were cleared rapidly from the renal tract. The renal virulence of the organisms was markedly enhanced by concomitant administration of ferric sorbitol citrate which resulted in extensive growth of the bacteria in the kidneys and development of a progressive pyelonephritis. Therapy with appropriate penicillins prevented development of infection and in the studies reported here a combination of ampicillin and cloxacillin demonstrated marked synergy against an infection caused by an ampicillin-resistant strain of E. coli.
KeywordsConcomitant Administration Kidney Homogenate Renal Tract Intravenous Inoculation Large Inoculum
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