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Immunochemical Study of the Structural Specificity of an Anti-Gentamycin Antiserum, Useful Also for Radioimmunoassay of Sisomycin

  • S. Jonsson
Part of the Chemotherapy book series (CT, volume 2)

Abstract

At this Department a piece of collaborative work has resulted in a new method for the radioimmunoassay of gentamycin levels in biological fluids (Jonsson et al., 1974 and 1975). It differs in two important respects from the techniques applied by the first two groups in the field (Lewis et al., 1972, Mahon et al., 1973). The most important difference lies in the choice of the radioactive tracer. Since there is no commercial source supplying a reproducible preparation of tritiated gentamycin, and since it is technically much simpler to measure gamma radiation than beta radiation, a large effort was put into establishing a method for the reproducible preparation of a conjugate of gentamycin and human serum albumin (HSA). This product is easily labelled with radioactive iodine, 125I, in a reproducible fashion without loss of the antigenic activity of the coupled gentamycin. The second difference lies in the choice of technique for the separation of antibody-bound and free antigen. We have applied a solid phase separation reagent of killed protein A-containing Staphylococcus aureus (Jonsson and Kronvall, 1974) with a high capacity for binding more than 90 per cent of the serum IgG of some mammals such as man and rabbit.

Keywords

Human Serum Albumin Collaborative Work Radioactive Iodine Radioactive Tracer Aminoglycoside Antibiotic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. S. Jonsson, G. Kahlmeter, T. Hallberg, and G. Kronvall, Communication to the VIth Int. Congr. of Inf. and Parasitic Diseases, Warszawa, Poland, Sept. 1974, Abstracts volume p. 323.Google Scholar
  2. S. Jonsson, G. Kahlmeter, T. Hallberg, and G. Kronvall (1975), Manuscript submitted for publication.Google Scholar
  3. S. Jonsson and G. Kronvall (1974), Europ. J. Immunol. 4: 29CrossRefGoogle Scholar
  4. J.E. Lewis, J.C. Nelson, and H.A. Elder (1972), Nature New Biology 239: 214.PubMedGoogle Scholar
  5. W.A. Mahon, J. Ezer, and T.W. Wilson (1973), Antimicrob. Agents Chemother. 3: 585PubMedCrossRefGoogle Scholar
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Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • S. Jonsson
    • 1
  1. 1.Department of Medical MicrobiologyUniversity of LundLundSweden

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