Punch Hole Method. A Simplified Bio-Assay Technique of Antibiotic Concentrations

  • Shigeru Kondo
Part of the Chemotherapy book series (CT, volume 2)


In the bio-assay of antibiotic concentrations in body fluids such as bile, blood, urine, etc., the diffusion of antibiotics into an agar medium is commonly utilized, and the techniques of these bio-assay are usually that of horizontal diffusion and that of vertical diffusion. In the former, the cup method, the paper cisc method, and the band culture method (okubo and Fujimoto, 1970 are the most commonly used, while in the latter, the super-position method (Torii, 1959) is the most common.


Agar Medium Paper Disc Plate Medium Antibiotic Concentration Vertical Diffusion 
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  1. Okubo, H. and Fujimoto, Y. (1970), Progress in Antimicrobial and Anticancer Chemotherapy,l, 495.Google Scholar

Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • Shigeru Kondo
    • 1
  1. 1.Osaka Medical CollegeTakatsuki, OsakaJapan

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