Assay of Gentamicin and Tobramycin by a Reliable 2½ Hour Klebsiella Plate Method
A 2½ hour Klebsiella plate diffusion method for serum gentamicin and tobramycin assays is described which involves increasing the incubation temperature to 40°C and the use of a heavy inoculum. The accuracy of the method compares favourably with that of the conventional 4 to 5 hours or overnight assays at 35°C. The advantage of the more rapid assay is that results can be given the same day on specimens which arrive in the late morning or early afternoon. Results would then be available before the next injection of aminoglycoside so that the dose can be adjusted accordingly.
KeywordsTest Serum Overnight Culture Zone Size Large Plate Small Plate
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