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Assay of Gentamicin and Tobramycin by a Reliable 2½ Hour Klebsiella Plate Method

  • D. C. Shanson
  • C. Hince
  • J. V. Daniels
Part of the Chemotherapy book series (CT, volume 2)

Summary

A 2½ hour Klebsiella plate diffusion method for serum gentamicin and tobramycin assays is described which involves increasing the incubation temperature to 40°C and the use of a heavy inoculum. The accuracy of the method compares favourably with that of the conventional 4 to 5 hours or overnight assays at 35°C. The advantage of the more rapid assay is that results can be given the same day on specimens which arrive in the late morning or early afternoon. Results would then be available before the next injection of aminoglycoside so that the dose can be adjusted accordingly.

Keywords

Test Serum Overnight Culture Zone Size Large Plate Small Plate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Noone, P., Pattison, J.R. and Garfield Davies, D. (1974) Postgraduate Medical Journal, 50 (Suppl. 7, 9–16.Google Scholar
  2. Noone, P. and Pattison, J.R. (1975) J. Clin.Path., 28, 83–84.PubMedCrossRefGoogle Scholar
  3. Reeves, D.S. (1972) Lancet, ii, 1369–1370.Google Scholar
  4. Shanson, D.C. (1975) Journal of Antimicrobial Chemotherapy, 1, 247–249.PubMedCrossRefGoogle Scholar
  5. Shanson, D.C. and Daniels, J.V. (1975) Journal of Antimicrobial Chemotherapy, 1, 219–227.PubMedCrossRefGoogle Scholar
  6. Waterworth, P.M. (1972) J. Clin. Path., 25, 979–983.Google Scholar

Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • D. C. Shanson
    • 1
  • C. Hince
    • 1
  • J. V. Daniels
    • 1
  1. 1.Department of Medical MicrobiologyThe London Hospital Medical CollegeLondonUK

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