The Problem of Antibiotic Mixtures in Serum Samples

  • D. S. Reeves
  • H. A. Holt
Part of the Chemotherapy book series (CT, volume 2)


The treatment of bacterial infection by the giving of more than one antibiotic simultaneously is a common and frequently justifiable practice. Clinical microbiological laboratories are therefore often faced with the problem of assaying an antibiotic in a sample of body fluid in the presence of one or more other antibiotics. Even when the antibiotics are given sequentially as treatment the residual presence of an antibiotic which has ceased being administered can prove an unexpected hazard to accurate assay. Microbiological assays are the commonest method used in clinical laboratories and although convenient they are usually lacking in specificity. This review will explore methods of increasing the specificity of microbiological assays and also briefly examine other more specific assays suitable for serum samples.


Cephal Exin Specific Assay Fusidic Acid Aminoglycoside Antibiotic Semi Carbazide 
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  1. Bratton, A.C., and Marshall, E.K. (1939). Journal of Biological Chemistry, 128: 537–50.Google Scholar
  2. Broughall, J.M. and Reeves, D.S. (1975b). Antimicrobial Agents and Chemotherapy. In press.Google Scholar
  3. Ervin, F.R., and Bullock, W.E. (1974). Ant imicrobial Agents and Chemotherapy, 6, 831–835.CrossRefGoogle Scholar
  4. Grove, D.C., and Randall, W.A. (1955). A Laboratory Manual. New York. Medical Encyclopaedia.Google Scholar
  5. Haas, M.J., and Davies, J. (1973). Antimi crobial Agents and Chemotherapy, 4, 497–499.CrossRefGoogle Scholar
  6. Konno, M., and Fujii, R. (1970). Progress in Antimicrobial and Anticancer Chemotherapy, Vol. 1, Baltimore University Park Press, 1027–1032.Google Scholar
  7. Lightbown, J.W., and de Rossi, P. (1965). Analyst, 70, 89–98.CrossRefGoogle Scholar
  8. Reeves, D.S., and Bywater, M.J. (1976). Balliere, Tindall and Cox, London. (In press).Google Scholar
  9. Reeves, D.S., and Holt, H.A. (1975a). Journal of Clinical Pathology (in press).Google Scholar
  10. Sabath, L.D., Casey, J.I., Ruch, P.A., Stumpf, L.L., and Finland, M. (1971). Journal of Laboratory of Clinical Medicine, 78, 457–463.Google Scholar
  11. Sabath, L.D., Loder, P.B., Gerstein, D.A., and Finland, M. (1968). Applied Microbiology. 16: 877–880.PubMedGoogle Scholar
  12. Schwartz, D.E., Koechlin, B.A., and Weinfeld, R.E. (1969). Chemotherapy, 14, 22–29.PubMedCrossRefGoogle Scholar
  13. Stroy, S.A., and Preston, D.A. (1971). Applied Microbiology, 21, 1002–1006.PubMedGoogle Scholar
  14. Waterworth, P.M. (1973). Journal of Clinical Pathology. 26, 596–598.PubMedCrossRefGoogle Scholar
  15. Zuidweg, M.H.J., Oostendorp, J.G., and Bos, C.J.K. (1969). Journal of Chromatography, 42, 552–554.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • D. S. Reeves
    • 1
  • H. A. Holt
    • 1
  1. 1.Department of Medical MicrobiologySouthmead HospitalBristolEngland

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