A New Assay Technique for Antibiotics
Endogenous ATP levels have been used as an index of bacterial growth in a rapid microbiological assay for aminoglycoside antibiotics. The test organism is Klebsiella edwardsii var atlantae (NCTC 10896) which is resistant to penicillins and cephalosporins. Bacterial ATP is extracted with a solution of EDTA in H2SO4 and measured using the firefly bioluminescence system. There is a close inverse relationship between bacterial ATP and serum antibiotic level. This assay technique is potentially applicable to any group of antimicrobial agents.
KeywordsTest Organism Aminoglycoside Antibiotic Assay Technique Standard Graph Serum Antibacterial Activity
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