A New Assay Technique for Antibiotics

  • M. J. Harber
  • A. W. Asscher
Part of the Chemotherapy book series (CT, volume 2)


Endogenous ATP levels have been used as an index of bacterial growth in a rapid microbiological assay for aminoglycoside antibiotics. The test organism is Klebsiella edwardsii var atlantae (NCTC 10896) which is resistant to penicillins and cephalosporins. Bacterial ATP is extracted with a solution of EDTA in H2SO4 and measured using the firefly bioluminescence system. There is a close inverse relationship between bacterial ATP and serum antibiotic level. This assay technique is potentially applicable to any group of antimicrobial agents.


Test Organism Aminoglycoside Antibiotic Assay Technique Standard Graph Serum Antibacterial Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Broughall, J.M. and Reeves, D.S. (1975), Journal of Clinical Pathology, 28, 140.PubMedCrossRefGoogle Scholar
  2. Conn, R. B., Carache, P. and Chappelle, E.W. (1975), American Journal of Clinical Pathology, 63, 493.PubMedGoogle Scholar
  3. D’Eustachio, A.J. and Johnson, D.R. (1968), Federation Proceedings, 27, 761.Google Scholar
  4. Faine, S. and Knight, D.C. (1968), Lancet, 2, 375.PubMedCrossRefGoogle Scholar
  5. Haas, M.J. and Davies, J. (1973), Antimicrobial Agents and Chemotherapy, 4, 497.PubMedCrossRefGoogle Scholar
  6. Lewis, J.E., Nelson, J.C. and Elder, H.A. (1972), Nature New Biology, 239, 214.PubMedGoogle Scholar
  7. McElroy, W. D. and Strehler, B. L. (1949), Archives of Biochemistry, 22, 420.PubMedGoogle Scholar
  8. Minshew, B. H., Holmes, R.K. and Baxter, C. R. (1975), Antimicrobial Agents and Chemotherapy, 7, 107.PubMedCrossRefGoogle Scholar
  9. Noone, P. Pattison, J.R. and Samson, D. (1971), Lancet, 2, 16. Reeves, D.S. (1974), Postgraduate Medical Journal, 50 (suppl. 7), 20.Google Scholar
  10. Smith, D. H., Van Otto, B. and Smith, A. L. (1972), New England Journal of Medicine, 286, 583.PubMedCrossRefGoogle Scholar
  11. St. John, J. B. (1970), Analytical Biochemistry, 37, 409.CrossRefGoogle Scholar
  12. Strehler, B. L. and Totter, J.R. (1952), Archives of Biochemistry and Biophysics, 40, 28.PubMedCrossRefGoogle Scholar
  13. Ten Krooden, E. and Darrell, J.H. (1974), Journal of Clinical Pathology, 27, 452.PubMedCrossRefGoogle Scholar
  14. Van Dyke, K., Stitzel, R., McClellen, T. and Szustkiewicz, C. (1969), Clinical Chemistry, 15, 3.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • M. J. Harber
    • 1
  • A. W. Asscher
    • 1
  1. 1.K. R. U. F. Institute of Renal Disease, Welsh National School of MedicineRoyal InfirmaryCardiffUK

Personalised recommendations