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A New Assay Technique for Antibiotics

  • M. J. Harber
  • A. W. Asscher
Part of the Chemotherapy book series (CT, volume 2)

Summary

Endogenous ATP levels have been used as an index of bacterial growth in a rapid microbiological assay for aminoglycoside antibiotics. The test organism is Klebsiella edwardsii var atlantae (NCTC 10896) which is resistant to penicillins and cephalosporins. Bacterial ATP is extracted with a solution of EDTA in H2SO4 and measured using the firefly bioluminescence system. There is a close inverse relationship between bacterial ATP and serum antibiotic level. This assay technique is potentially applicable to any group of antimicrobial agents.

Keywords

Test Organism Aminoglycoside Antibiotic Assay Technique Standard Graph Serum Antibacterial Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • M. J. Harber
    • 1
  • A. W. Asscher
    • 1
  1. 1.K. R. U. F. Institute of Renal Disease, Welsh National School of MedicineRoyal InfirmaryCardiffUK

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