Fluorimetric Assay of Tetracycline Mixtures in Plasma
A fluorimetric technique is described for the analysis of mixtures of tetracyclines in plasma. The method is capable of accurately measuring the individual levels of each tetracycline present in the therapeutic range, to as low as 0.1 mg/L or less. In operation it is simple and inexpensive to perform, consisting of only two or three basic steps: (1) Conversion of the tetracyclines into a fluorescent form by simple acid hydrolysis, or where appropriate, by alkaline degradation, (2) intensification of the fluorescence, where necessary, by complexation with aluminium, and (3) measurement in a spectrofluorimeter.
KeywordsTherapeutic Range Peak Excitation Chemical Alteration Measurement Wavelength FLUORIMETRIC Assay
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