Fluorimetric Assay of Tetracycline Mixtures in Plasma

Part of the Chemotherapy book series (CT, volume 2)


A fluorimetric technique is described for the analysis of mixtures of tetracyclines in plasma. The method is capable of accurately measuring the individual levels of each tetracycline present in the therapeutic range, to as low as 0.1 mg/L or less. In operation it is simple and inexpensive to perform, consisting of only two or three basic steps: (1) Conversion of the tetracyclines into a fluorescent form by simple acid hydrolysis, or where appropriate, by alkaline degradation, (2) intensification of the fluorescence, where necessary, by complexation with aluminium, and (3) measurement in a spectrofluorimeter.


Therapeutic Range Peak Excitation Chemical Alteration Measurement Wavelength FLUORIMETRIC Assay 
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    Boothe, J.H., Morton II, J., Petisi, J.P., Wilkinson, R.G., Williams, J.H. (1953) J. Am. Chem. Soc., 75, 4621.Google Scholar
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Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • D. Hall
    • 1
  1. 1.Dept. of Clinical PharmacologySt. Bartholomew’s HospitalLondonUK

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