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Two-Dimensional Electrophoresis with Silver Staining: A Sensitive Method For Analyzing Complex Protein Mixtures

  • Marcia F. Goldfarb
Conference paper
Part of the Lecture Notes on Coastal and Estuarine Studies book series (COASTAL, volume 25)

Abstract

About 10 years ago, O’Farrell combined two electrophoretic methods which separated proteins by different parameters to produce a greatly enhanced separation of proteins in serum. This idea was developed by Anderson and Anderson (1978a) at Argonne Laboratory, and they have called their methodology the Iso-Dalt system (Anderson et al., 1978a; Anderson et al., 1978b).

Keywords

Human Milk Spot Pattern Western Blot Technique Electrophoretic Method Human Milk Sample 
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References

  1. Anderson, N.G. and N.L. Anderson. 1978a. Analytical Techniques for cell fractions. XXI. Two-dimensional analysis of serum and tissue proteins: Multiple isoelectric focusing. Anal. Biochem. 85: 331–340.PubMedCrossRefGoogle Scholar
  2. Anderson, N.L. and N.G. Anderson. 1978b. Analytical techniques for cell fractions. XXII. Two-dimensional analysis of serum and tissue proteins: Multiple gradient-slab electrophoresis. Anal. Biochem. 85: pp 334–354.Google Scholar
  3. Merril, C.R., D. Goldman, and M.L. Van Keuren. 1982. Simplified silver protein detection and image enhancement methods in Polyacrylamide gels. Electrophoresis 3: 17–23.CrossRefGoogle Scholar
  4. Symington, J. 1984. Electrophoretic transfer of proteins from two-dimensional gels to sheets and their detection. IN: Two dimensional gel electrophoresis of proteins. Celis, Bravo, (ed.). Academic Press, pp 127–168.Google Scholar
  5. Clinical Chemistry. 1982. Part II, 28(4): pp 737–1092.Google Scholar
  6. Clinical Chemistry. 1984. Part I, 30(2): pp 1897–2108.Google Scholar

Copyright information

© Springer-Verlag New York, Inc. 1988

Authors and Affiliations

  • Marcia F. Goldfarb
    • 1
  1. 1.Anatek-EPPortlandUSA

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