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Preparation and use of Monospecific Antisera to Capitella SP. I.

  • Eugene D. Gallagher
  • J. P. Grassle
  • D. D. Trueblood
  • M. G. Gleason
Part of the Lecture Notes on Coastal and Estuarine Studies book series (COASTAL, volume 25)

Abstract

The opportunistic polychaetes formerly known as Capitella capitata are the archetypal pioneers in soft-bottom benthic succession. Capitella is now known to be comprised of several morphologically indistinguishable but reproductively isolated sibling species. Capitella sp. I is widely distributed on the east coast of the United States and has been used in a number of experimental and toxicological studies. We used three immunoelectrophoretic techniques (i.e., 2-dimensional, Krøll’s line, and Laurell’s rocket electrophoresis) to identify and purify a dominant antigen in this species. To study the role of Capitella species in field experiments, we developed a monospecific antiserum to this major antigen. We plan to use this monospecific typing serum and FITC-immunocytochemical staining to identify the formalin-preserved larvae and juveniles of Capitella sp. I. This immunocytochemical staining technique will enable us to test processes controlling the distribution and abundance of Capitella sp. I in the field.

Keywords

United State Infectious Disease Field Experiment East Coast Toxicological Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer-Verlag New York, Inc. 1988

Authors and Affiliations

  • Eugene D. Gallagher
    • 1
  • J. P. Grassle
    • 2
  • D. D. Trueblood
    • 3
  • M. G. Gleason
    • 4
  1. 1.Environmental Sciences ProgramUniversity of Massachusetts, BostonBostonUSA
  2. 2.Marine Biological LaboratoryWoods HoleUSA
  3. 3.Environmental Sciences ProgramUniversity of Massachusetts, BostonBostonUSA
  4. 4.Department of ZoologyUniversity of CaliforniaBerkeleyUSA

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