Preparation and use of Monospecific Antisera to Capitella SP. I.

  • Eugene D. Gallagher
  • J. P. Grassle
  • D. D. Trueblood
  • M. G. Gleason
Part of the Lecture Notes on Coastal and Estuarine Studies book series (COASTAL, volume 25)


The opportunistic polychaetes formerly known as Capitella capitata are the archetypal pioneers in soft-bottom benthic succession. Capitella is now known to be comprised of several morphologically indistinguishable but reproductively isolated sibling species. Capitella sp. I is widely distributed on the east coast of the United States and has been used in a number of experimental and toxicological studies. We used three immunoelectrophoretic techniques (i.e., 2-dimensional, Krøll’s line, and Laurell’s rocket electrophoresis) to identify and purify a dominant antigen in this species. To study the role of Capitella species in field experiments, we developed a monospecific antiserum to this major antigen. We plan to use this monospecific typing serum and FITC-immunocytochemical staining to identify the formalin-preserved larvae and juveniles of Capitella sp. I. This immunocytochemical staining technique will enable us to test processes controlling the distribution and abundance of Capitella sp. I in the field.


United State Infectious Disease Field Experiment East Coast Toxicological Study 
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Copyright information

© Springer-Verlag New York, Inc. 1988

Authors and Affiliations

  • Eugene D. Gallagher
    • 1
  • J. P. Grassle
    • 2
  • D. D. Trueblood
    • 3
  • M. G. Gleason
    • 4
  1. 1.Environmental Sciences ProgramUniversity of Massachusetts, BostonBostonUSA
  2. 2.Marine Biological LaboratoryWoods HoleUSA
  3. 3.Environmental Sciences ProgramUniversity of Massachusetts, BostonBostonUSA
  4. 4.Department of ZoologyUniversity of CaliforniaBerkeleyUSA

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