Immunochemical Evaluation of The Infectious Pancreatic Necrosis (IPN) Carrier State in Salmonids and Other Species

  • Paul W. Reno
Conference paper
Part of the Lecture Notes on Coastal and Estuarine Studies book series (COASTAL, volume 25)


One of the most serious and pervasive diseases of cultured salmonids is infectious pancreatic necrosis (IPN) which is caused by an unusual virus of the same name (IPNV). The disease is especially prevalent on the West coast of the U.S. and the Canadian Maritime Provinces, but is also found in Asia, the British Isles and Europe. More than fifty species of fish and shellfish have been determined to harbor the virus (Dorson, 1983), although overt disease is not a necessary consequence of infection. When disease does occur, however, it is lethal to a large proportion of the affected population; this has been documented in many species including Atlantic salmon, Salmo salar; Pacific salmon, Oncorhyncus sp.; striped bass, Morone saxitilis; menhaden, Brevoortia tyrranus; rainbow trout, Salmo gairdneri; brook trout, Salvelinis fontinalis; and eels, Anguilla sp. Isolation of the virus from asymptomatic animals has been accomplished with at least twenty other aquatic animals, including freshwaster, estuarine, and marine vertebrates and invertebrates. Among the more intriguing species infected are shrimp, hard clam, American and European oyster, blue crab, minnows, milkfish and tilapia (Dorson,1983).


Striped Bass Blue Crab Infectious Virus Brook Trout Normal Rabbit Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Caswell-Reno, P., P.W. Reno, and B.L. Nicholson. 1986. Monoclonal Antibodies to Infectious Pancreatic Necrosis Virus (IPNV): Analysis of Viral Epitopes and Comparison of Different Isolates. J. Gen. Virol. 67: 2193–2205.PubMedCrossRefGoogle Scholar
  2. Dorson, M. 1983. Infectious Pancreatic Necrosis. IN: Antigens of Fish Pathogens. D. Anderson, M. Dorson, and P. Dubourget, (eds.). Collection Fond. M. Merieux.Google Scholar
  3. Frantsi, C. and M. Savan. 1971. Infectious Pancretic Necrosis: Comparative Frequencies of Isolation from Feces and Organs of Brook Trout, Salvelinus fontinalis. J. Fish. Res. Bd. Can. 26: 1064–1065.CrossRefGoogle Scholar
  4. Kelly, R. and P. Loh. 1972. Electron Microscopical and Biochemical Characterization of Infectious Pancreatic Necrosis Virus. J. Virol. 10: 824–834.PubMedGoogle Scholar
  5. Nakane, P. and A. Kawaoi. 1974. Peroxidase-labelled Antibody, a New Method of Conjugation. J. Histo. Cyto. 22: 1084–1091.CrossRefGoogle Scholar
  6. Nicholson, B.L. and E. Henchal. 1978. Rapid Identification of Infectious Pancreatic Necrosis virus in Infected Cell Cultures by Immunoperoxidase Techniques. J. Wild. Dis. 14: 465–469.Google Scholar
  7. Reno, P.W. 1976. Qualitative and Quantitative Aspects of Infectious Pancreatic Necrosis Carrier State. Ph. D. Thesis, University of Guelph, Ontario.Google Scholar
  8. Weir, E.E., T. Pretlow, A. Pitts, E. Williams. 1974. A More Sensitive and Specific Histochemical Peroxidase Stain for the Localization of Cellular Antigen by the Enzyme-antibody Conjugate Method. J. Histo. Cyto. 22: 51.CrossRefGoogle Scholar
  9. Wolf, K. and M.C. Quimby. 1962. Established Eurythermic Line of Fish Cells In Vitro. Sci. 135: 1065–1066.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1988

Authors and Affiliations

  • Paul W. Reno
    • 1
  1. 1.Department of Microbiology and Migratory Fish Research InstituteUniversity of MaineOronoUSA

Personalised recommendations