Enzyme Immunoassay Utilizing Monoclonal Antibodies for Identification of European Eel Virus (EEV), an Aquatic Birnavirus
European eel virus (EEV) is the etiological agent of branchio-nephritis, one of the most serious diseases of eels in Asian fish culture (Sano et al., 1981). This virus was first isolated from the European eel (Anguilla anguilla) and termed EEV; later, it was also isolated from the Japanese eel (A. japonica). Subsequently, EEV has been isolated from both healthy and diseased eels throughout Japan and Taiwan (Kou et al., 1983; Sano et al., 1981). Also, a virus similar, and possibly identical, to EEV has been isolated from Tilapia in Taiwan (Kou et al., 1983). Experimental infection with EEV produced high levels of mortality in eels and Tilapia sp. but not in rainbow trout.
KeywordsInfectious Pancreatic Necrosis Virus Oxalacetic Acid Infected Cell Culture Serum Free Growth Medium CsCl Gradient Centrifugation
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