A Possible Immunological Approach to the Study of Plasmalemma Redox Enzymes in Phytoplankton
The phytoplankton plasmalemma contains redox enzymes which will reduce external trace metal complexes, redox dyes and molecular oxygen (Jones and Morel, 1986; Jones et al., 1985; Palenik et al., 1986). The chemical properties of the trace metal reductase are consistent with those of NAD(P)H:cytochrome c (ferricyanide) reductase which is found as a prosthetic group in many cellular redox enzymes e.g., nitrate reductase, sulfite reductase and oleate desaturase. We are now attempting to substantiate these initial observations through the use of anti-serum, prepared against algal nitrate reductase, as a probe for the plasmalemma trace metal reductase. Preliminary experiments indicate that nitrate reductase anti-serum completely inhibits plasmalemma redox activity in the marine diatoms Thalassiosira weissflogii and T. pseudonana.
KeywordsTrace Metal Molecular Oxygen Nitrate Reductase Prosthetic Group Redox Enzyme
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