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A Possible Immunological Approach to the Study of Plasmalemma Redox Enzymes in Phytoplankton

  • Gary J. Jones
  • Francois M. M. Morel
  • Clarice M. Yentsch
  • Edward A. Funkhouser
Conference paper
Part of the Lecture Notes on Coastal and Estuarine Studies book series (COASTAL, volume 25)

Abstract

The phytoplankton plasmalemma contains redox enzymes which will reduce external trace metal complexes, redox dyes and molecular oxygen (Jones and Morel, 1986; Jones et al., 1985; Palenik et al., 1986). The chemical properties of the trace metal reductase are consistent with those of NAD(P)H:cytochrome c (ferricyanide) reductase which is found as a prosthetic group in many cellular redox enzymes e.g., nitrate reductase, sulfite reductase and oleate desaturase. We are now attempting to substantiate these initial observations through the use of anti-serum, prepared against algal nitrate reductase, as a probe for the plasmalemma trace metal reductase. Preliminary experiments indicate that nitrate reductase anti-serum completely inhibits plasmalemma redox activity in the marine diatoms Thalassiosira weissflogii and T. pseudonana.

Keywords

Trace Metal Molecular Oxygen Nitrate Reductase Prosthetic Group Redox Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Jones, G.J., B. Palenik and F.M.M. Morel. 1987. Trace metal reduction by phytoplankton: the roll of plasmalemma redox enzymes. J. Phycol. (in press)Google Scholar
  2. Jones, G.J., T.D. Waite and J.D. Smith. 1985. Light-dependent reduction of copper (II) and its effect on cell-mediated, thiolo-dependent superoxide production. Biochem. Biophys. Res. Comm. 128: 1031–1036.PubMedCrossRefGoogle Scholar
  3. Palenik, B., O.C. Zafiriou and F.M.M. Morel. 1987. Hydrogen peroxide production by a marine phytoplankton. Limnol. Oceanogr. (submitted)Google Scholar

Copyright information

© Springer-Verlag New York, Inc. 1988

Authors and Affiliations

  • Gary J. Jones
    • 1
  • Francois M. M. Morel
    • 1
  • Clarice M. Yentsch
    • 2
  • Edward A. Funkhouser
    • 3
  1. 1.Massachusetts Institute of Technology 48-213CambridgeUSA
  2. 2.Bigelow Laboratory for Ocean SciencesWest Boothbay HarborUSA
  3. 3.Department of BiochemistryTexas A & M UniversityCollege StationUSA

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