Abstract
An immunological approach for the examination of low molecular weight lipid polyether marine toxins in fish tissues was initiated in 1977 with the development of a modified radioimmunoassay (RIA) using anti-ciguatoxin antibody prepared in sheep (Hokama et al., 1977). Subsequently, a solid phase enzyme immunoassay (EIA) using the same sheep anti-CTX was established (Hokama et al., 1983; Hokama et al., 1984). The EIA procedure permitted the evaluation of cross-reactivity between ciguatoxin (CTX), okadiac acid (OA), brevetoxin (PbTX), maitotoxin (MTX) and monensin by competitive EIA using these purified toxins (Hokama et al., 1984). In 1984, Baden et al., developed a competitive radioimmunoassay for assessment of PbTX. More recently (Baden et al., 1985), using this same RIA procedure and goat anti-PbTX the cross-reactivity of PbTX and CTX was demonstrated.
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References
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Hokama, Y., Honda, S.A.A., Kobayashi, M.N., Nakagawa, L.K., Shirai, L.K., Miyahara, J.T. (1988). Monoclonal Antibodies (Monoabs) to Ciguatoxin and Related Polyethers. In: Yentsch, C.M., Mague, F.C., Horan, P.K. (eds) Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions. Lecture Notes on Coastal and Estuarine Studies, vol 25. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-7642-2_11
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DOI: https://doi.org/10.1007/978-1-4684-7642-2_11
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