Monoclonal Antibodies (Monoabs) to Ciguatoxin and Related Polyethers
An immunological approach for the examination of low molecular weight lipid polyether marine toxins in fish tissues was initiated in 1977 with the development of a modified radioimmunoassay (RIA) using anti-ciguatoxin antibody prepared in sheep (Hokama et al., 1977). Subsequently, a solid phase enzyme immunoassay (EIA) using the same sheep anti-CTX was established (Hokama et al., 1983; Hokama et al., 1984). The EIA procedure permitted the evaluation of cross-reactivity between ciguatoxin (CTX), okadiac acid (OA), brevetoxin (PbTX), maitotoxin (MTX) and monensin by competitive EIA using these purified toxins (Hokama et al., 1984). In 1984, Baden et al., developed a competitive radioimmunoassay for assessment of PbTX. More recently (Baden et al., 1985), using this same RIA procedure and goat anti-PbTX the cross-reactivity of PbTX and CTX was demonstrated.
KeywordsHuman Serum Albumin Ammonium Sulfate Okadaic Acid Fish Tissue Coated Bead
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