Similarity between Earthworm Cuticle Collagen and the Chemoattractant for Garter Snakes Solubilized from the Exterior Surface of Earthworms
When earthworms (EW, Lumbricus terrestris) are washed for 1 minute at 60C° with water a substance is solubilized which is a powerful chemoattractant (CA) for garter snakes1. Dialysis against water through 3500 MW pore size dialysis tubing and lyophilization yields a fluffy, white, water-soluble (20 mg/2 ml) product. This product, EWDL, has absorption at 280 nm, gives positive Lowry and Bradford tests for protein, and contains neutral sugar. When EWDL is separated on a column of either G-75 or AcA 44, two peaks, detected by 280 nm absorption are obtained. One peak, F2, contains a substance with molecular weight greater than 65,000 and has all the CA activity. The other peak, F4, contains a substance with a much smaller molecular weight, less than 12,000, and is devoid of CA activity2,3.
KeywordsSmall Molecular Weight Exterior Surface Neutral Sugar Garter Snakes1 Ninhydrin Assay
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