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Similarity between Earthworm Cuticle Collagen and the Chemoattractant for Garter Snakes Solubilized from the Exterior Surface of Earthworms

  • Donald M. Kirschenbaum
  • Patti T. Yao
  • Nancy Schulman
  • Mimi Halpern
Part of the NATO ASI Series book series (NSSA, volume 93)

Abstract

When earthworms (EW, Lumbricus terrestris) are washed for 1 minute at 60C° with water a substance is solubilized which is a powerful chemoattractant (CA) for garter snakes1. Dialysis against water through 3500 MW pore size dialysis tubing and lyophilization yields a fluffy, white, water-soluble (20 mg/2 ml) product. This product, EWDL, has absorption at 280 nm, gives positive Lowry and Bradford tests for protein, and contains neutral sugar. When EWDL is separated on a column of either G-75 or AcA 44, two peaks, detected by 280 nm absorption are obtained. One peak, F2, contains a substance with molecular weight greater than 65,000 and has all the CA activity. The other peak, F4, contains a substance with a much smaller molecular weight, less than 12,000, and is devoid of CA activity2,3.

Keywords

Small Molecular Weight Exterior Surface Neutral Sugar Garter Snakes1 Ninhydrin Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Donald M. Kirschenbaum
    • 1
  • Patti T. Yao
    • 1
  • Nancy Schulman
    • 2
  • Mimi Halpern
    • 2
  1. 1.Department of BiochemistryDonstate Medical CenterBrooklynUSA
  2. 2.Department of Anatomy and Cell BiologyDonstate Medical CenterBrooklynUSA

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