Muscarinic Acetylcholine Receptor-Linked Inositide Cycle in the Central Nervous System

  • Lucio A. A. van Rooijen
  • Jörg Traber
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 221)


The inositide cycle (Fig.1) constitutes a second messenger system mediating the cellular response to activation of particular receptors (for reviews: Abdel-Latif, 1983; Berridge, 1984; Fisher et al., 1984a; Nishizuka, 1984). It is generally accepted that the initial event following receptor activation is phosphodiesteratic degradation of phosphatidylinositol 4, 5-bisphosphate (PIP2) to diacylglycerol (DG) and D-myo-inositol 1, 4, 5-trisphosphate (IP3). The latter is degraded by specific phosphatases eventually to myo-inositol. DG is readily phosphorylated to phosphatidate (PA), which is converted through a liponucleotide-intermediate to phosphatidylinositol (PI). In two sequential phosphorylation steps, PI is converted to PIP2, via phosphatidylinositol 4-phosphate (PIP), thus closing the inositide cycle. One way to analyze receptor-sensitive operation of the inositide cycle is to measure the appearance of labeled inositol phosphates in [3H]-inositol (pre)incubated cells. The use of Li+, which inhibits the last step of the degradation of IP3 to myo-inositol (Hallcher and Sherman, 1980), has proven valuable in this analysis (Berridge et al., 1982). Alternatively one can measure concurrent labeling of phospholipids from 32Pi. Receptor activation will then enhance incorporation of radiotracer into PA and PI.


Muscarinic Receptor Nerve Ending Muscarinic Acetylcholine Receptor Inositol Trisphosphate Inositol Phospholipid 
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© Plenum Press, New York 1987

Authors and Affiliations

  • Lucio A. A. van Rooijen
    • 1
  • Jörg Traber
    • 1
  1. 1.Neurobiology DepartmentTroponwerke GmbH and Co. KGCologne 80Federal Republic of Germany

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