Gene Expression and Interferon
The regulation of gene expression in the interferon system falls into two distinct sub-topics. First is the induction of the interferon structural genes in cells infected with a virus or treated with some other inducer, such as double-stranded RNA or lectins. The second involves the mechanism of interferon action, in which several distinct genes are induced in interferon-treated cells to be transcribed and translated as the cells become virus resistant or cease their usual division cycle. In neither the induction of interferon nor its action is much known about specific effects on chromatin structure, but it is presumed that perturbations do occur as in other examples of regulated gene expression. Besides providing yet another “model” system, the interferons offer the following features: very rapid alterations in gene expression initiated at the cell surface by a relatively few inducing molecules (only one molecule in some cell types), leading to profound biological sequelae (refractility to viruses and blockade of cell cycle movements). The interferons are particularly interesting because cell division often stops in treated cells, in comparison to other hormonally-regulated systems where division rates are enhanced. The property of interferons to make cell cultures quiescent thus avoids the problem of studying cascades of gene expression which occur in growth-stimulated cells.
KeywordsHuman Interferon Human Fibroblast Cell Interferon Gene Interferon Receptor Intracellular Polyamine
Unable to display preview. Download preview PDF.
- 1.LEBLEU, B. and CONTENT, J. (1982). Mechanisms of interferon action: biochemical and genetic approaches. In: “Interferon 4,” ( I. Gresser, ed.) p. 47. Academic Press, New York.Google Scholar
- 2.MARCUS, P. (1983). Interferon induction by viruses: one molecule of dsRNA as the threshold for induction. In: “Interferon 5,” ( I. Gresser, ed.) p. 115. Academic Press, New York.Google Scholar
- 4.BARON, S. (1984). Overview of progress in interferon research, 1979–1983. In: “Interferon: Research, Clinical Application, and Regoa atory Consideration,” ( K. Zoon, P. Noguchi, and T. Liu, eds.) p. 3. Elsevier, New York.Google Scholar
- 5.REVEL, M. (1983). Genetic and functional diversity of interferons in man. In: “Interferon 5,” ( I. Gresser, ed.) p. 206. Academic Press, New York.Google Scholar
- 8.DERYNCK, R. (1983). More about interferon cloning. In:“Interferon 5,” (I. Gresser, ed.) p. 181. Academic Press, New York.Google Scholar
- 15.AGUET, M. and MOGENSEN, K. (1983). In: “Interferon 5,” (I. Gresser, ed.) P. 1. Academic Press, New York.Google Scholar
- 16.JOSHI, A., SARKAR, F., and GUPTA, S. (1982). Crosslinking of human leukocyte interferon α-2 to its receptor on human cells. J. Biol. Chem. 257, 3884.Google Scholar
- 20.U.S. patents 4,311,639; 4,341,761; and 4,438,030.Google Scholar
- 26.LEE, E. and SREEVALSAN, T. (1981). Interferon as an inhibitor of polyamine enzymes. In: “Advances in Polyamine Research,” ( C. Caldera, ed.) p. 175. Raven Press, New York.Google Scholar