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Confocal Laser Microscopy and Digital Image Analysis in Microbial Ecology

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Book cover Advances in Microbial Ecology

Part of the book series: Advances in Microbial Ecology ((AMIE,volume 12))

Abstract

Microbial ecologists have extensively explored the potential applications of light microscopy for more than five decades (Henrici and Johnson, 1935; Perfil’ev and Gabe, 1969; Casida, 1969, 1972, 1975, 1976; Staley, 1971; Caldwell and Hirsch, 1973; Caldwell et al., 1973, 1975; Caldwell and Tiedje, 1975a,b; Labeda et al., 1976; Hirsch, 1977, 1980; Geesey et al., 1978; Marshall, 1986). Now traditional microscopy has given way to “microvisualization” (Friedhoff, 1991) greatly accelerating research. Microorganisms are no longer merely photographed; instead, they are digitally “imaged” using fluorescent molecular probes, confocal laser microscopy, and computer image analysis. The chemical and biological relationships between a microorganism and its microenvironment are seen directly, nondestructively, in situ, and in “real time” (Lawrence and Caldwell, 1990). Consequently, it is no longer necessary to disrupt microbial communities when studying the molecular or behavioral aspects of their ecology.

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Authors and Affiliations

  1. Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 0W0, Canada

    Douglas E. Caldwell & Darren R. Korber

  2. National Hydrology Research Institute, Environment Canada, Saskatoon, Saskatchewan, S7N 3H5, Canada

    John R. Lawrence

Authors
  1. Douglas E. Caldwell
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  2. Darren R. Korber
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  3. John R. Lawrence
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Editor information

Editors and Affiliations

  1. University of New South Wales, Kensington, New South Wales, Australia

    K. C. Marshall

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© 1992 Plenum Press, New York

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Caldwell, D.E., Korber, D.R., Lawrence, J.R. (1992). Confocal Laser Microscopy and Digital Image Analysis in Microbial Ecology. In: Marshall, K.C. (eds) Advances in Microbial Ecology. Advances in Microbial Ecology, vol 12. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7609-5_1

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  • DOI: https://doi.org/10.1007/978-1-4684-7609-5_1

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-7611-8

  • Online ISBN: 978-1-4684-7609-5

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