The Nature of Intramembraneous Particles

  • A. J. Verkleij
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 71)


Freeze-fracturing electron microscopy has become a very popular method in membrane biology over the last decade and it is also of great value in the study of the structural details of aqueous lipid dispersions. This can simply be attributed to a faithful physical fixation method and the replica method, both of which exclude the artifacts frequently introduced in this section (positive staining) and negative staining electron microscopy (Zingsheim, 1972). At present freeze fracturing is technically straightforward and the reproducibility is high. Moreover, the fracturing process intrinsic to the method is now reasonably well understood (Verkleij and Ververgaert, 1978).


Erythrocyte Membrane Fracture Face Freezing Method Freeze Fracture INTRAMEMBRANEOUS Particle 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • A. J. Verkleij
    • 1
  1. 1.Institute of Molecular BiologyState University of UtrechtUtrechtThe Netherlands

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