The Nature of Intramembraneous Particles
Freeze-fracturing electron microscopy has become a very popular method in membrane biology over the last decade and it is also of great value in the study of the structural details of aqueous lipid dispersions. This can simply be attributed to a faithful physical fixation method and the replica method, both of which exclude the artifacts frequently introduced in this section (positive staining) and negative staining electron microscopy (Zingsheim, 1972). At present freeze fracturing is technically straightforward and the reproducibility is high. Moreover, the fracturing process intrinsic to the method is now reasonably well understood (Verkleij and Ververgaert, 1978).
KeywordsErythrocyte Membrane Fracture Face Freezing Method Freeze Fracture INTRAMEMBRANEOUS Particle
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- Branton, D., 1966, Fracture faces of frozen membranes, Proc. Natl. Acad. Sci. USA, 55: 1048.Google Scholar
- Bullivant, S., 1974, Membranes, freeze-etching techniques applied to biological membranes, Phil. Trans. Roy. Soc. Lond. B, 268:Google Scholar
- Dupont, Y., Gabriel, A., Chabre, M., Gulik-Krzywicki, T. and Schechter, E., 1972, Use of new detector for X-ray diffraction and kinetics of the ordering of the lipids in E. coli membranes and model systems, Nature, 238: 331.Google Scholar
- Müller, M., Meister, N. and Moor, H., 1980, Freezing in a propane-jet and its application in freeze fracturing, Mikroskopie (Wien) 36: 129.Google Scholar
- Zingsheim, H.P., 1972, Membrane structure and electron microscopy. The significance of physical problems and technics (freeze-etching), Biochim. Biophys. Acta, 265: 339.Google Scholar
- Zingsheim, H.P. and Plattner, H., 1976, Electron microscopic methods in membrane biology, in: “Methods in Membrane Biology”, Vol. 7, ( Korn, E.D., ed.), Plenum Publ. Co., New York, p. 1–146.Google Scholar